Comprehensive profile and contrastive analysis of circular RNA expression in cervical squamous carcinoma and adenocarcinoma

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Bioinformatics and Genomics

Main article text

 

Introduction

Materials & Methods

Specimens preparation

RNA extraction and quality control

Library building for RNA-seq

Bioinformatic analysis

Quantitative real-time PCR

Results

General expression profiles of circRNAs in different types of cervical cancer

Confirmation of results with RNA-seq for differentially expressed circRNAs

Comprehensive analysis of function enrichment derived from different comparisons

Relative expression level detection of key candidate circRNAs

Construction of a ceRNA network based on candidate circRNAs and their host genes

Discussion

Conclusions

Supplemental Information

Supplemental table and files

DOI: 10.7717/peerj.14759/supp-1

Additional Information and Declarations

Competing Interests

The authors declare there are no competing interests.

Author Contributions

Hongxue Luo conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Yi Zhu performed the experiments, analyzed the data, prepared figures and/or tables, and approved the final draft.

Jiaqi Wang performed the experiments, prepared figures and/or tables, and approved the final draft.

Yue Wang conceived and designed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Lihui Wei analyzed the data, authored or reviewed drafts of the article, and approved the final draft.

Human Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

The ethics committee of Peking university people’s hospital approved the study (2019PHB212-01).

Data Availability

The following information was supplied regarding data availability:

The raw sequencing data are available at NCBI’s Gene Expression Omnibus (GEO): GSE208089.

Funding

The study was supported by the National Natural Science Foundation of China (Grant No. 81903053) and Peking University People’s Hospital Scientific Research Development Funds (RDY2019-07). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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