Antimicrobial and anti-inflammatory activity of Cystatin C on human gingival fibroblast incubated with Porphyromonas gingivalis

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RT @blancas_esther: My article has been published today in @PeerJLife https://t.co/gGJa5TD4YU #Biochemistry #CellBiology #Microbiology #Den…
Biochemistry, Biophysics and Molecular Biology

Main article text

 

Introduction

Materials & Methods

Cells culture

Bacterial growth

Antibacterial assay

Cell viability assay

Treatment of human gingival fibroblasts (HGFs) with P. gingivalis

Cytokine assays

Measurement of NO production

Detection of Reactive Oxygen Species (ROS)

Statistical analysis

Results

Effects of cystatin C on growth of P. gingivalis and viability of HGFs

Effect of cystatin C on the production of pro- and anti-inflammatory cytokines

Cystatin C decreases ROS and NO production on HGFs incubated with P. gingivalis

Discussion

Conclusions

Supplemental Information

Raw data used for the figures

DOI: 10.7717/peerj.14232/supp-1

Effect of cystatin C in Porphyromonas gingivalis growth and cell viability

Effect of cystatin C on a) Porphyromonas gingivalis growth and b) Cell viability of HGFs. The results are expressed as mean ± SD of the average of five independent assays. Statistical differences are expressed as (*) p < 0.05 when compared to non-treated control P. gingivalis bacteria

DOI: 10.7717/peerj.14232/supp-2

Additional Information and Declarations

Competing Interests

The authors declare there are no competing interests.

Author Contributions

Blanca Esther Blancas-Luciano conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Ingeborg Becker-Fauser conceived and designed the experiments, analyzed the data, authored or reviewed drafts of the article, and approved the final draft.

Jaime Zamora-Chimal conceived and designed the experiments, performed the experiments, authored or reviewed drafts of the article, and approved the final draft.

José Delgado-Domínguez conceived and designed the experiments, performed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Adriana Ruíz-Remigio conceived and designed the experiments, performed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Elba Rosa Leyva-Huerta conceived and designed the experiments, analyzed the data, authored or reviewed drafts of the article, and approved the final draft.

Javier Portilla-Robertson conceived and designed the experiments, analyzed the data, authored or reviewed drafts of the article, and approved the final draft.

Ana María Fernández-Presas conceived and designed the experiments, performed the experiments, analyzed the data, authored or reviewed drafts of the article, and approved the final draft.

Data Availability

The following information was supplied regarding data availability:

The raw data is available in the Supplemental Files.

Funding

This work was supported by grant #IN218419 from PAPITT, DGAPA, UNAM, Mexico City, and by Universidad Anahuac México Campus Norte. Blanca Esther Blancas-Luciano is supported by CONACYT grant #424031 for her doctoral studies. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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