Signal peptidase 21 suppresses cell proliferation, migration, and invasion via the PTEN-PI3K/Akt signaling pathway in lung adenocarcinoma

View article
Bioinformatics and Genomics

Main article text

 

Introduction

Materials & Methods

Analysis in silico

Tissue samples

Immunohistochemistry (IHC)

Cell culture

siRNA transfection

RT-qPCR

Western blotting

Cell proliferation assay

5-Ethynyl-2′ -deoxyuridine (EdU) assay

Plate colony formation

Transwell migration and invasion assays

Wound healing assay

Statistical analysis

Results

SPC21 mRNA expression is upregulated in LUAD and related with good prognosis

SPC21 suppresses proliferation, invasion, and migration in LUAD cells

SPC21 inhibits the PI3K/Akt pathway through PTEN in LUAD cells

Discussion

Conclusions

Supplemental Information

Original data for tables

DOI: 10.7717/peerj.14206/supp-1

Original data for figures

DOI: 10.7717/peerj.14206/supp-2

(A) The protein-protein interaction network of differential expressed proteins in LUAD analyzed by String. (B)The connection degree of protein-protein interaction analysis showed by Pajek

DOI: 10.7717/peerj.14206/supp-3

WB original images

DOI: 10.7717/peerj.14206/supp-4

Additional Information and Declarations

Competing Interests

The authors declare there are no competing interests.

Author Contributions

Na Zhang conceived and designed the experiments, analyzed the data, prepared figures and/or tables, and approved the final draft.

Shiguang Cao performed the experiments, prepared figures and/or tables, and approved the final draft.

Ruiying Sun performed the experiments, prepared figures and/or tables, and approved the final draft.

Yibei Wang performed the experiments, prepared figures and/or tables, and approved the final draft.

Luna Liu performed the experiments, prepared figures and/or tables, and approved the final draft.

Wei Wang conceived and designed the experiments, analyzed the data, authored or reviewed drafts of the article, and approved the final draft.

Xia Meng conceived and designed the experiments, analyzed the data, authored or reviewed drafts of the article, and approved the final draft.

Human Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

The Institutional Review Board of the Second Affiliated Hospital of Xi’an Jiaotong University approval to carry out the study within its facilities (Ethical Application Ref: 2021084).

Data Availability

The following information was supplied regarding data availability:

The original western blotting gels images are available in the Supplemental Files.

Funding

This work was supported by the Natural Science Foundation of Shaanxi Province (No. 2022JQ-906) and the Free Orientation Fund of the Second Affiliated Hospital of Xi’an Jiaotong University (No. 2020YJ(ZYTS)439). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

3 Citations 775 Views 99 Downloads

Your institution may have Open Access funds available for qualifying authors. See if you qualify

Publish for free

Comment on Articles or Preprints and we'll waive your author fee
Learn more

Five new journals in Chemistry

Free to publish • Peer-reviewed • From PeerJ
Find out more