A novel alkane monooxygenase (alkB) clade revealed by massive genomic survey and its dissemination association with IS elements

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Bioinformatics and Genomics

Main article text

 

Introduction

Materials and Methods

Identification of alk-gene cluster

Phylogeny of alk-gene cluster-carrying genomes

Phylogeny of the alkB genes

Organization structure of alk-genes

Results

Phylogenetic diversity of genomes carrying alk-gene clusters

Niches distribution of strains carrying alk-gene clusters

Alk-fragments distribution on genome

Phylogenetic diversity of alkB from alk-fragments

Organization diversity of alk-genes and IS elements distribution

Discussion

Conclusion

Supplemental Information

Niches distribution of strains carrying alk-gene clusters along with the corresponding phylogenetic tree

The phylogenetic tree was the same as in Fig. 1. The niches distribution is represented by the Check Mark on the right.

DOI: 10.7717/peerj.14147/supp-1

Alk-fragments distribution on genome

DOI: 10.7717/peerj.14147/supp-2

Taxonomic distribution of all available genomes included in this analysis

The number of genomes from the top 15 phyla (A) and top 15 genera (B) was represented by bar size.

DOI: 10.7717/peerj.14147/supp-3

The GenBank assembly accession number of 117 genomes

DOI: 10.7717/peerj.14147/supp-4

Newick tree files corresponding to Figure 1

DOI: 10.7717/peerj.14147/supp-5

Newick tree files corresponding to Figure 2

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The accession numbers of prokaryotes genomes used in this study

DOI: 10.7717/peerj.14147/supp-7

Additional Information and Declarations

Competing Interests

The authors declare there are no competing interests.

Author Contributions

Shaojing Wang conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Guoqiang Li conceived and designed the experiments, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Zitong Liao performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Tongtong Liu performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Ting Ma conceived and designed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Data Availability

The following information was supplied regarding data availability:

The prokaryotes genomes (both bacteria and archaea) accession numbers are available in the Supplementary File.

Funding

This study was supported by the National Key Research and Development Plan (2018YFA0902101) and the NSFC project (42173079). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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