WRKY transcription factor family in lettuce plant (Lactuca sativa): Genome-wide characterization, chromosome location, phylogeny structures, and expression patterns

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Bioinformatics and Genomics

Main article text

 

Introduction

Material and Methods

Sequence retrieval and identification of WRKY TFs in lettuce

Gene structure, conserved motif, and cis-elements analysis of promoter

Multiple sequence alignment and phylogenetic tree of LsWRKY TFs

Chromosomal distribution and syntenic relationship of LsWRKY TFs

Plant materials, stress and phytohormone treatments

Quantitative transcript analysis and qRT-PCR validation

Statistical analysis

Results

Identification of LsWRKY TFs in lettuce

Multiple sequence alignment and phylogenetic analysis of LsWRKY TFs

Gene structure, conserved motif, and cis-elements analysis of LsWRKY TFs

Chromosomal distribution and syntenic relationship of LsWRKY TFs

Interaction network analysis of LsWRKY TFs

Expression patterns of LsWRKYIII subfamily genes in response to abiotic stress

Salt stress

Drought stress

Low temperature (4 °C)

High temperature (37 °C)

Expression patterns of LsWRKYIII subfamily genes under treatment with phytohormone

Tissue-specific expression patterns of LsWRKYIII subfamily genes

Transcript abundance analysis of LsWRKY genes in lettuce

Validation of expression profile of LsWRKYIII subfamily genes at different stages of stem enlargement

Discussion

Identification of WRKY TFs family in lettuce

Phylogenetic analysis of LsWRKY TFs

Roles of LsWRKY TFs in abiotic stress

Roles of LsWRKY TFs in plant growth and development

Regulation mechanism of WRKY TFs involved in different biological processes

Conclusion

Supplemental Information

Alignment of the amino acid sequence of LsWRKY TFs

The red box represented the conservative domain WRKYGQK of WRKY TFs; amino acids of different colors indicated different degrees of similarity.

DOI: 10.7717/peerj.14136/supp-1

WRKY family TFs members among different plant species

Different colored boxes represented different WRKY subfamily TFs.

DOI: 10.7717/peerj.14136/supp-2

Phylogenetic analysis of WRKY TFs lettuce and Arabidopsis by MEGA7.0

Different colors represent different subfamily WRKY TFs.

DOI: 10.7717/peerj.14136/supp-3

Supplemental tables

DOI: 10.7717/peerj.14136/supp-4

Additional Information and Declarations

Competing Interests

The authors declare there are no competing interests.

Author Contributions

Ping Du performed the experiments, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Qinglian Wu performed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Yihua Liu performed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Xue Cao analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Wenyi Yi analyzed the data, authored or reviewed drafts of the article, and approved the final draft.

Tikun Jiao performed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Mengqi Hu analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Ying Huang conceived and designed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Data Availability

The following information was supplied regarding data availability:

The raw measurements are available in the Supplemental Files.

Funding

This work was supported by: the National Natural Science Foundation of China: 32102405, 31801868, and the Natural Science Foundation of Shandong Province: ZR2020QC156, ZR2020QC063. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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