Identification and expression profile analysis of the SnRK2 gene family in cucumber

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Bioinformatics and Genomics

Main article text

 

Introduction

Material and Methods

Plant materials

Identification of CsSnRK2 genes

Chromosome localization and basic information analysis

Collinearity analysis of CsSnRK2 genes

Phylogenetic, structure and conserved motif analysis

Analysis of cis-acting elements, tissue-specific expression and protein interaction networks

RNA extraction and Real-Time PCR (qRT-PCR)

Results

Chromosome distribution and basic information of CsSnRK2 genes

Collinearity and selective pressure analysis of the CsSnRK2 genes

Phylogenetic tree analysis of the CsSnRK2 genes

Structure and conserved motif analysis of the CsSnRK2 genes

Cis-acting element of the CsSnRK2 promoter region

Tissue-specific expression analysis of the CsSnRK2 genes

qRT-PCR expression characteristics of the CsSnRK2 genes

Analysis of the protein interaction network of the CsSnRK2 family genes

Discussion

Conclusions

Supplemental Information

Protein sequences

DOI: 10.7717/peerj.13994/supp-1

The original CT value of qRT-PCR

DOI: 10.7717/peerj.13994/supp-2

Additional Information and Declarations

Competing Interests

The authors declare there are no competing interests.

Author Contributions

Zilong Wan performed the experiments, analyzed the data, prepared figures and/or tables, and approved the final draft.

Shilei Luo conceived and designed the experiments, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Zeyu Zhang performed the experiments, analyzed the data, prepared figures and/or tables, and approved the final draft.

Zeci Liu analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Yali Qiao analyzed the data, prepared figures and/or tables, and approved the final draft.

Xueqin Gao analyzed the data, prepared figures and/or tables, and approved the final draft.

Jihua Yu analyzed the data, prepared figures and/or tables, and approved the final draft.

Guobin Zhang conceived and designed the experiments, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Data Availability

The following information was supplied regarding data availability:

The raw data is available in the Supplementary File.

Funding

This research was supported by Director Foundation of Key Laboratory of Arid Habitat Crop Science of Gansu Province, China (GHSJ-2020-Z3) and Education science and technology innovation project of Gansu Province (2021CYZC-45). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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