Ferroptotic cardiomyocyte-derived exosomes promote cardiac macrophage M1 polarization during myocardial infarction

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Just published in PeerJ Life & Environment - Ferroptotic cardiomyocyte-derived exosomes promote cardiac macrophage M1 polarization during myocardial infarction Read the full article https://t.co/5h4z57sLpK #Biochemistry #CellBiology #Cardiology
Just published in PeerJ Life & Environment - Ferroptotic cardiomyocyte-derived exosomes promote cardiac macrophage M1 polarization during myocardial infarction Read the full article https://t.co/rGcGJE6XQ7 #Biochemistry #CellBiology #Cardiology
Biochemistry, Biophysics and Molecular Biology

Main article text

 

Introduction

Materials and Methods

Mouse model of MI

Echocardiographic examination

Hematoxylin-Eosin (H&E) staining

Ferroptosis indicators measurement

Western blotting

Cell culture and treatment

Isolation and identification of exosome

Uptake of exosomes

Flow cytometry sorting of macrophages

Phagocytosis of macrophages

MiRNA mimics and siRNA transfection

RT-qPCR analysis

Statistical analysis

Results

Cardiomyocyte ferroptosis accompanied by M1 macrophage infiltration during MI

Ferroptotic cardiomyocytes derived exosome identification

Ferroptotic cardiomyocytes derived exosome promote macrophage M1 polarization

Ferroptotic cardiomyocytes derived exosome repaired phagocytosis of RAW 264.7 cells

Ferroptotic cardiomyocytes derived exosome promote macrophage M1 polarization via miR-106b-3p

Discussion

Conclusions

Supplemental Information

Author Checklist - Full

DOI: 10.7717/peerj.13717/supp-1

Raw data for LVIDs, EF, FS, MDA, Fe2+, protein expression, M2 macrophages, phagocyte cell counts, full-length uncropped blots

DOI: 10.7717/peerj.13717/supp-2

The primers used in this study

DOI: 10.7717/peerj.13717/supp-3

Additional Information and Declarations

Competing Interests

The authors declare there are no competing interests.

Author Contributions

Shengjia Sun conceived and designed the experiments, performed the experiments, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Yurong Wu analyzed the data, prepared figures and/or tables, and approved the final draft.

Alimujiang Maimaitijiang analyzed the data, authored or reviewed drafts of the article, and approved the final draft.

Qingyu Huang performed the experiments, prepared figures and/or tables, and approved the final draft.

Qiying Chen conceived and designed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

Animal Welfare and Ethics Group, Department of Experimental Animal Science, Fudan University (2020 Huashan Hospital JS-574).

Data Availability

The following information was supplied regarding data availability:

The raw data is available in the Supplemental File.

Funding

The authors received no funding for this work.

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