Search for morphological indicators that predict implantation by principal component analysis using images of blastocyst

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Biochemistry, Biophysics and Molecular Biology

Main article text

 

Introduction

Methods

Ethics statement

Patient selection and cycle characteristics

IVF and ET protocols and medications

Pregnancy tests

Principal component analysis

Gardner scoring

Statistical analysis

Results

PCA using blastocyst images

Blastocyst graininess was the highlighted morphological parameter

Feature of non-grainy blastocysts

Visual classification of blastocyst by graininess improves implantation (hCG positive) prediction accuracy

Discussion

Supplemental Information

Non-grainy blastocyst was caused by fragmentation during the early cleavage stage.

(A) A typical image showing the production of non-grainy blastocyst. (B) Illustrated process showing the production of non-grainy blastocyst.

DOI: 10.7717/peerj.13441/supp-2

Raw data: P-value of statistical analysis and contribution rate.

Results of statistical analysis on each PC, contribution rate, and cumulative contribution ratio.

DOI: 10.7717/peerj.13441/supp-3

A typical video showing how the non-grainy blastocyst was produced.

Fragmentation was observed in the early cleavage stage, while cellular debris was observed during the morula stage.

DOI: 10.7717/peerj.13441/supp-5

Additional Information and Declarations

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Daisuke Mashiko conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Mikiko Tokoro performed the experiments, analyzed the data, authored or reviewed drafts of the paper, and approved the final draft.

Masae Kojima performed the experiments, analyzed the data, authored or reviewed drafts of the paper, and approved the final draft.

Noritaka Fukunaga conceived and designed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Yoshimasa Asada conceived and designed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Kazuo Yamagata conceived and designed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Human Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

Analysis of human embryo movies was carried out with the approval of the Ethics Committee of Asada Ladies Clinic (Approval number: 2021-04), Kindai University (approval number: R2-1-001), and the Japanese Society of Obstetrics and Gynecology (date of approval: 06/30/2021).

Data Availability

The following information was supplied regarding data availability:

The raw measurements are available in the Supplemental Files.

Funding

This work was supported by the JSPS KAKENHI, with grant numbers JP25712035, JP25116005, JP18H05528, and JP18H02357 to Kazuo Yamagata. Daisuke Mashiko is a JSPS Research Fellow (202100155). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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