Skin microbiota diversity among genetically unrelated individuals of Indian origin

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Potbhare et al. @UniTurku examine #skin #microbiota diversity among genetically unrelated individuals of Indian origin Read the full article https://t.co/4x43vZVp6u #Biodiversity #Microbiology #Dermatology https://t.co/b7p2PyTfOc
Potbhare et al. @UniTurku examine #skin #microbiota diversity among genetically unrelated individuals of Indian origin Read the full article https://t.co/qoQxRTuuCH #Biodiversity #Microbiology #Dermatology https://t.co/6MiuvcfSkU
Microbiology

Main article text

 

Introduction

Materials and Methods

Human ethics committee

Subject selection

Sample collection

DNA extraction

16S rRNA gene amplicon sequencing

Illumina library preparation and sequencing

Assembly of sequence data

Statistical analysis

Results

Core microbiota

Alpha diversity

Beta diversity

Differential genus abundance

Discussion

Conclusion

Supplemental Information

The detected total number of bacterial reads (abundances) as per Operational Taxonomic Unit (OTUs) across each sample

DOI: 10.7717/peerj.13075/supp-1

Bacterial taxonomic classification

List of bacterial taxonomic classification based on obtained sequences with respect to Operational Taxonomic Unit (OTUs).

DOI: 10.7717/peerj.13075/supp-2

Additional Information and Declarations

Competing Interests

The authors declare there are no competing interests.

Author Contributions

Renuka Potbhare performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper and approved the final draft.

Ameeta RaviKumar, Eveliina Munukka and Richa Ashma conceived and designed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Leo Lahti conceived and designed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Human Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

The present study has been approved by the Human Ethics Committee of Savitribai Phule Pune University (Letter no: SPPU/IEC/2019/60 Date: 20/11/2019), which follows the rules of the Indian Council of Medical Research (ICMR), India.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

The samples used here are available at the European Nucleotide Archive: ERS6234946 to ERS6235003; PRJEB44216.

Data Availability

The following information was supplied regarding data availability:

The raw data are available in the Supplementary Files.

The source code for the analysis is available under the open-source MIT license: Renuka Potbhare, & Leo Lahti. (2021). Article_2021_sparc (1.01). Zenodo. https://doi.org/10.5281/zenodo.5805004.

Funding

This work was supported by the Scheme for Promotion of Academic and Research Collaboration (SPARC), under the Ministry of Human Resource Development (MHRD), Government of India (GOI) Scheme (SPARC/2018-2019/P794/SL; dated –20/03/2019) and Academy of Finland (decision 295741; to LL). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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