Microbial community spatial structures in Luzhou-flavored liquor pit muds with different brewing materials

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Microbiology

Main article text

 

Introduction

Materials and Methods

Sample collection

DNA extraction and high-throughput sequencing

Data analysis

Results

Discussion

Conclusions

Supplemental Information

Process flow chart introducing the detailed brewing process of Luzhou-flavored Baijiu.

The black triangle indicates the phase of sampling.

DOI: 10.7717/peerj.12987/supp-1

Principle components analysis profile based on weighted UniFrac distances of pit mud microbial communities.

DOI: 10.7717/peerj.12987/supp-2

Partial redundancy analysis results showing the contributions of pit location, and brewing materials to the microbial community difference.

DOI: 10.7717/peerj.12987/supp-3

Linear discriminant analysis effect size result comparing pit mud microbial communities between M3G and M5G.

DOI: 10.7717/peerj.12987/supp-4

Cladogram plot showing the LEfSe results of pit mud microbial communities among different layers.

DOI: 10.7717/peerj.12987/supp-5

LEfSe results of pit mud microbial communities among different layers.

DOI: 10.7717/peerj.12987/supp-6

Additional Information and Declarations

Competing Interests

Lei Zheng is employed by Shandong Baimai Spring Wine Co., Ltd., China. Ru Jiang is employed by Shandong Yinlu Food Co., Ltd., China.

Author Contributions

Jinjin Li conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Hongzhao Sun conceived and designed the experiments, performed the experiments, analyzed the data, authored or reviewed drafts of the paper, and approved the final draft.

Qian Wang performed the experiments, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Yunfei Cai performed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Zhu Shi performed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Jianlei Jia performed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Lei Zheng performed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Ru Jiang performed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Lingmei Gao analyzed the data, authored or reviewed drafts of the paper, and approved the final draft.

Data Availability

The following information was supplied regarding data availability:

All merged sequences are available in the Genome Sequence Archive database: https://bigd.big.ac.cn/gsa/browse/CRA004711.

Funding

This research was funded by the Doctoral Launching Project of Qilu Normal Univeristy, grant number BS2017008. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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