Identification of high protein kinase CK2α in HPV(+) oropharyngeal squamous cell carcinoma and correlation with clinical outcomes

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Introduction

Materials and methods

Patients

p16, Ki-67 and CK2 IHC stain

E6 and E7 RNA in situ hybridization stain

Definition of HPV status

Human HNSCC TCGA data analysis

Statistical analysis

Survival analysis

Results

Patient and tumor characteristics overall

CK2α staining and association with patient characteristics

Evaluation of CK2α protein levels in tumors and correlations with HPV status and Ki-67 index

Survival analysis

Survival analysis in TCGA HPV(−) HNSCC

Discussion

Conclusions

Supplemental Information

OPSCC Clinical and Biomarker Data

DOI: 10.7717/peerj.12519/supp-1

IHC Full Size Images

DOI: 10.7717/peerj.12519/supp-2

Statistical analyses and codebook

DOI: 10.7717/peerj.12519/supp-3

Additional Information and Declarations

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Janeen H. Trembley conceived and designed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Bin Li conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Betsy T. Kren and Khalil Ahmed conceived and designed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Justin Peltola performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Juan Manivel performed the experiments, analyzed the data, authored or reviewed drafts of the paper, and approved the final draft.

Devi Meyyappan performed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Amy Gravely analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Mark Klein and Emiro Caicedo-Granados conceived and designed the experiments, analyzed the data, authored or reviewed drafts of the paper, and approved the final draft.

Human Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

The study was approved by the Institutional Review Board of Minneapolis VA Health Care System. Data analysis was completed under an approved Research and Development Committee protocol.

Data Availability

The following information was supplied regarding data availability:

The deidentified clinical and biomarker data, complete IHC images and statistical analyses are available in the Supplementary Files.

Funding

This work was supported by Merit Review research funds I01 BX003282 and I01 BX005091 awarded by the United States Department of Veterans Affairs Biomedical Laboratory Research and Development Service (Khalil Ahmed); Lion’s award (Bin Li); private donation (Emiro Caicedo-Granados). There was no additional external funding received for this study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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