Zoledronic acid promotes osteoclasts ferroptosis by inhibiting FBXO9-mediated p53 ubiquitination and degradation

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Biochemistry, Biophysics and Molecular Biology

Main article text

 

Introduction

Materials and Methods

Cell culture

TRAP staining

Measurement of cell viability

Fe2+ concentration

Lipid reactive oxygen species (ROS) assay

MDA and GSH content

Transient transfection of FBXO9 or si-FBXO9

Quantitative real-time PCR (qPCR)

Western blotting analysis

Co-immunoprecipitation

Statistical analysis

Results

ZA treatment facilitated the ferroptosis of osteoclasts

FBXO9 was downregulated in osteoclasts after ZA treatment

FBXO9 inhibition facilitated the ferroptosis of osteoclasts

ZA treatment facilitated the ferroptosis of osteoclasts by suppressing FBXO9

FBXO9 inhibition facilitated the ferroptosis of osteoclasts by blocking the ubiquitin mediated-proteasome degradation of p53

Discussion

Conclusions

Supplemental Information

Raw data for non-WB.

DOI: 10.7717/peerj.12510/supp-1

Raw data for WB.

DOI: 10.7717/peerj.12510/supp-2

Additional Information and Declarations

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Xingzhou Qu performed the experiments, prepared figures and/or tables, and approved the final draft.

Zhaoqi Sun analyzed the data, authored or reviewed drafts of the paper, and approved the final draft.

Yang Wang conceived and designed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Hui Shan Ong conceived and designed the experiments, prepared figures and/or tables, and approved the final draft.

Data Availability

The following information was supplied regarding data availability:

The raw data is available in the Supplemental Files.

Funding

The authors received no funding for this work.

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