Circular RNA RBM33 contributes to extracellular matrix degradation via miR-4268/EPHB2 axis in abdominal aortic aneurysm

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Bioinformatics and Genomics

Main article text

 

Introduction

Materials & methods

Tissue samples

RNA extraction, library construction and sequencing

RT-qPCR

Western Blot

Luciferase reporter assay

Statistical analysis

Results

Analysis of DEcircRNAs

Functional and pathway analysis of DEcircRNAs

circRBM33 is highly expressed in AAA

Overexpression of circRBM33 induces the ECM degradation

circRBM33 mediates the ECM degradation by directly binding to miR-4268

circRBM33 regulates EPHB2 expression

Discussion

Conclusions

Supplemental Information

Venn diagram of circRNAs in AAA tissues and adjacent normal tissues.

DOI: 10.7717/peerj.12232/supp-1

Primer sequences for RT-qPCR.

DOI: 10.7717/peerj.12232/supp-2

Primer sequences for circHLA-RBM33.

DOI: 10.7717/peerj.12232/supp-3

Summary statistics of RNA sequencing data.

AAA: AAA group. Con: control group (normal tissues).

DOI: 10.7717/peerj.12232/supp-4

Raw data for RT-qPCR.

DOI: 10.7717/peerj.12232/supp-5

The full length images of western blot results for Figs. 4C, 5F, 6C.

DOI: 10.7717/peerj.12232/supp-6

Supplementary method.

DOI: 10.7717/peerj.12232/supp-7

Additional Information and Declarations

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Shizhi Wang performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, methodology, and approved the final draft.

Qingwen Yuan performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Wenpeng Zhao performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Weimin Zhou conceived and designed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Human Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

The Ethics Committee of the Second Affiliated Hospital of Nanchang University approval to carry out the study within its facilities (Ethical Application Ref: Study Clinical Review (2020) No. 44).

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

The RNA sequences described here are accessible via GenBank accession number PRJNA699407.

Data Availability

The following information was supplied regarding data availability:

The data is available at figshare: Zhou, Weimin (2021): Data Files. figshare. Dataset. https://doi.org/10.6084/m9.figshare.16441944.v3.

The raw data for RT-qPCR and the western blots are available as Supplemental Files.

Funding

This work was supported by the Science and Technology Project of the Education Department of Jiangxi province (GJJ200197). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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