Exosomal hsa-miR-21-5p is a biomarker for breast cancer diagnosis

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Exosomal hsa-miR-21-5p is a biomarker for breast cancer diagnosis https://t.co/sH99T3U6GC @thePeerJ https://t.co/1sOrY0EJYp
Bioinformatics and Genomics

Main article text

 

Introduction

Materials & Methods

Microarray dataset acquisition

Identifying DE-miRNAs and DE-mRNAs

Predicting DE-miRNAs targeting mRNAs and acquisition of overlapping mRNAs

Functional enrichment analysis

Constructing the PPI and miRNA-mRNA network

Expressing potential target genes and drawing a Kaplan-Meier curve

Sample collection

Cell culture

Isolating and identifying exosomes

Western blotting

Reverse transcription and quantitative polymerase chain reaction (RT-qPCR) analysis

ROC curve analysis of the most significant difference in miRNA performance in BC diagnosis

Statistical analysis

Results

Identifying DE-miRNAs and DE-mRNAs

Predicting target genes and overlapping mRNA acquisition

Functional enrichment analysis of overlapping mRNAs

Constructing the PPI and miRNA-mRNA networks

hsa-miR-21-5p expression was significantly up-regulated in BC tissue and cells

Exosome identification

Elevated plasma hsa-miR-21-5p was tumor-derived and packaged into exosomes

Exosomal hsa-miR-21-5p was significantly up-regulated in BC patient plasma and had remarkable diagnostic value

Down-regulated expression of target genes TGFβR3 and EGFR in BC

TGFβR3 is associated with overall survival (OS) in BC patients

Discussion

Conclusion

Supplemental Information

52 overlapping mRNAs

DOI: 10.7717/peerj.12147/supp-3

GO and KEGG results

DOI: 10.7717/peerj.12147/supp-4

MiRNA-mRNA network

DOI: 10.7717/peerj.12147/supp-6

The primers used for the real-time PCR

DOI: 10.7717/peerj.12147/supp-8

Additional Information and Declarations

Competing Interests

The authors declare there are no competing interests.

Author Contributions

Min Liu conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Fei Mo, Xiaohan Song and Yan Yuan analyzed the data, prepared figures and/or tables, and approved the final draft.

Yun He performed the experiments, analyzed the data, authored or reviewed drafts of the paper, and approved the final draft.

Jiaoyan Yan and Ye Yang performed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Jian Huang and Shu Zhang conceived and designed the experiments, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Human Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

The Ethics Committee of the Affiliated Hospital of Guizhou Medical University approval to carry out the study within its facilities (Ethical Application NO. 2019032K and NO. 2019033K).

Data Availability

The following information was supplied regarding data availability:

The raw data are available at GEO: GSE29044 and GSE97811.

Funding

This project is supported by the National Natural Science Foundation of China (No.81860723 and 81960589), the Postdoctoral Science Foundation of China (No.2018M643862), the Science and Technology Fund of Guizhou Provincial Health and Family Planning Commission (No. gzwjkj2018-1-073), Science and Traditional Chinese Medicine, National Medicine Science and Technology Fund (No. QZYY-2018-2019) and Guizhou Science and Technology Plan Project (Guizhou Science and Technology Support [2021] General 097). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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