Cbl upregulates cysH for hydrogen sulfide production in Aeromonas veronii

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Microbiology

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Introduction

Materials & methods

Bacterial strains, plasmids and culture conditions

Complemented strain construction

H2S detection

RNA extraction and qRT-PCR

Fluorescence analysis

Bacterial one-hybrid analysis

Protein expression and purification

Electrophoretic mobility shift assay (EMSA)

Transcriptome analysis

Statistical analyses

Results

Transcriptomic analysis

The production of H2S was increased in the absence of SmpB under nutritionally deficient conditions

Cbl affects the generation of H2S

Cbl promotes the transcription of cysH

Cbl regulates downstream cysH by binding to the P cysJIH

Determination of the binding region of P cysJIH with Cbl protein

Discussion

Conclusions

Supplemental Information

The information about the strains, plasmids and primers used in this study.

DOI: 10.7717/peerj.12058/supp-1

RNA-sequencing of smpB-dependent gene expression in A. Veronii C4.

DOI: 10.7717/peerj.12058/supp-2

Raw Data of qRT-PCR, H2S detection and Fluorescence detection.

DOI: 10.7717/peerj.12058/supp-3

Additional Information and Declarations

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Yidong Zhang conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Zebin Liu conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, and approved the final draft.

Yanqiong Tang conceived and designed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Xiang Ma conceived and designed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Hongqian Tang conceived and designed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Hong Li conceived and designed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Zhu Liu conceived and designed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Data Availability

The following information was supplied regarding data availability:

The raw measurements are available in the Supplemental File and at NCBI: PRJNA493739.

Funding

This work was supported by the grants from the National Natural Science Foundation of China Nos. 31772887 (to Zhu Liu). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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