In vitro effect of resin infiltrant on resistance of sound enamel surfaces in permanent teeth to demineralization

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Introduction

Materials & Methods

Tooth selection and sample preparation

Microhardness measurement

Resin infiltration and polishing

Artificial demineralization

Staining and confocal laser scanning microscopy observation

Double-fluorescence staining

Simulated toothbrushing

Scanning electron microscopy observation

Statistical analysis

Results

Surface microhardness values of specimens in two groups

Confocal laser scanning microscopy results

Scanning electron microscopy results

Discussion

Conclusions

Supplemental Information

Microhardness, the depth of demineralization and the depth of penetration

DOI: 10.7717/peerj.12008/supp-1

Scanning electron microscopy supplementary picture

DOI: 10.7717/peerj.12008/supp-2

Staining supplementary picture

DOI: 10.7717/peerj.12008/supp-3

Double-fluorescence staining supplementary picture

DOI: 10.7717/peerj.12008/supp-4

Additional Information and Declarations

Competing Interests

The authors declare there are no competing interests.

Author Contributions

Meng Li, Zhengyan Yang and Yajing Huang performed the experiments, analyzed the data, prepared figures and/or tables, and approved the final draft.

Yueheng Li and Zhi Zhou conceived and designed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Human Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

The Stomatological Hospital of Chongqing Medical University granted ethical approval to carry out the study within its facilities (CQHS-REC-2018(LS No. 22).

Data Availability

The following information was supplied regarding data availability:

Data are available as Supplementary Files.

Funding

This work was supported by the Chongqing Science and Technology Comission (No.CSTC2019jcyj-msxmx0191) and the Chongqing Municipal Health Commission (No.2018MSXM036, No.2018QNXM023, and No.2017ZDXM018). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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