CGRP overexpression does not alter depression-like behavior in mice

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CGRP overexpression does not alter depression-like #behavior in mice https://t.co/MBaj46C7l4 @thePeerJ https://t.co/57RAWQXatu
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Biochemistry, Biophysics and Molecular Biology

Main article text

 

Introduction

Materials & methods

Animals

Social defeat stress (SDS) paradigm

Behavioral assessments

Open field test

Social interaction test

Sucrose preference test

Analysis of the CGRP levels

Quantitative analysis by real-time PCR

Western blotting

Statistical analysis

Results

Characterization of CGRP-overexpressing transgenic mice

CGRP-overexpressing mice display stress sensitivity as wild-type mice

Stress exposure stimuli inhibited the Akt-mTOR-p70S6K signaling pathway in CGRP-overexpressing mice hippocampi

Discussion

Conclusions

Supplemental Information

Raw data of Figure 1.

Effect size analysis was performed by Cohen’s d test.

DOI: 10.7717/peerj.11720/supp-1

Raw data of Fig. 3.

The detection was carried using ImageJ. The values obtained from wild-type mice were set to 100%.

DOI: 10.7717/peerj.11720/supp-3

ARRIVE 2.0 Checklist.

DOI: 10.7717/peerj.11720/supp-4

Full length blot of Fig. 3B.

Upper gel is BDNF. Bottom gel is Actin.

DOI: 10.7717/peerj.11720/supp-5

Full length blot of Fig. 3C.

Upper gel is phosphorylated-Akt. Bottom gel is Akt.

DOI: 10.7717/peerj.11720/supp-6

Additional Information and Declarations

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Narumi Hashikawa-Hobara conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Ami Otsuka performed the experiments, analyzed the data, prepared figures and/or tables, and approved the final draft.

Chihiro Okujima performed the experiments, prepared figures and/or tables, and approved the final draft.

Naoya Hashikawa performed the experiments, analyzed the data, authored or reviewed drafts of the paper, and approved the final draft.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

Institute Okayama University of Science provided full approval for this research (2016-03, 2017-08, 2018-04, 2019-08, 2020-08, 1,421, 1,562).

Data Availability

The following information was supplied regarding data availability:

Raw data is available in the Supplemental Files.

Funding

The authors received no funding for this work.

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