Influence of the dental topical application of a nisin-biogel in the oral microbiome of dogs: a pilot study

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Microbiology

Main article text

 

Introduction

Materials & methods

Animals’ selection

Nisin-biogel preparation

Nisin-biogel application and oral sampling

DNA extraction

Amplification of 16S rRNA

Sequencing and data processing

Statistical analysis

Results

Animal’s selection

Sequencing data

Diversity analysis

Taxonomic diversity

Species abundance

Discussion

Conclusions

Supplemental Information

Distribution of demultiplexed reads and reads used in taxa classification by sample and by timepoint.

T1—samples collected before nisin-biogel application; T2—after one application of the nisin-biogel; T3—after three applications of the nisin-biogel. SD—standard deviation; ID—identification.

DOI: 10.7717/peerj.11626/supp-1

Raw material regarding Filum and Species identifcation.

Description of the number of reads obtained by sample and taxonomic level. Filum and Species were the taxonomic levels evaluated.

DOI: 10.7717/peerj.11626/supp-2

Additional Information and Declarations

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Eva Cunha conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, and approved the final draft.

Sara Valente performed the experiments, prepared figures and/or tables, and approved the final draft.

Mariana Nascimento performed the experiments, prepared figures and/or tables, and approved the final draft.

Marcelo Pereira analyzed the data, prepared figures and/or tables, and approved the final draft.

Luís Tavares conceived and designed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Ricardo Dias conceived and designed the experiments, analyzed the data, authored or reviewed drafts of the paper, and approved the final draft.

Manuela Oliveira conceived and designed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

Animals were included in this study after the owner’s written consent and approval by the Ethical Committee for Research and Teaching (CEIE) of the Faculty of Veterinary Medicine—University of Lisbon, Portugal (N/Refª 014/2020).

Data Availability

The following information was supplied regarding data availability:

The distribution of demultiplexed reads and reads used in taxa classification by sample and by timepoint, and the datasets used, are available in the Supplemental Files.

The original sequence datasets are available at NCBI SRA: PRJNA703322.

Funding

This research was funded by CIISA—Centro de Investigação Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade de Lisboa, Lisboa, Portugal, Project UIDB/00276/2020 (funded by FCT), and by the Foundation for Science and Technology (Eva Cunha PhD fellowship SFRH/BD/131384/2017). There was no additional external funding received for this study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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