Wnt signaling related transcripts and their relationship to energy metabolism in C2C12 myoblasts under temperature stress

View article
Biochemistry, Biophysics and Molecular Biology

Main article text

 

Introduction

Materials & methods

Cell culture

Heat/cold stress treatment

Bioenergetics assay

Quantitative real-time expression

Total membranes isolations

Western blot analysis

Data analysis

Results

Transcripts related Wnt signaling under cold/heat stress

Expression pattern of Wnt signaling genes and mitochondrial/ glycolysis functions

Wnt signaling genes and their relationship to energy metabolism after exposure to Cold/Heat stress

Temperature stress induces phosphorylation of LRP6 on cell membranes

Discussion

Transcripts related Wnt/PCP, Wnt/Ca2+ and Wnt/β-catenin under cold/heat stress

Wnt signaling genes and their relationship to energy metabolism after exposure to Cold/Heat stress

Conclusion

Supplemental Information

Raw data of the RT-PCR data

Each temperature was performed independently for three replicates, each replicate was performed in three wells, and each sample (well) was performed in two technical replicates.

DOI: 10.7717/peerj.11625/supp-1

Original Western blot image for total protein and correspondence blot of LPR6 protein from gel 1, gel 2, and gel 3

All Figure was carried using ChemiDoc Imaging Systems (Bio-rad). We used the total protein amount as a reference to normalize LPR6 protein data. Western blot analysis was performed in three independent experiments.

DOI: 10.7717/peerj.11625/supp-2

Raw data of Fig. 2

The detection was carried using ChemiDoc Imaging Systems (Bio-rad). We used the total protein amount as a reference to normalize LPR6 protein data. Western blot analysis was performed in three independent experiments.

DOI: 10.7717/peerj.11625/supp-3

Additional Information and Declarations

Competing Interests

The authors declare there are no competing interests.

Author Contributions

Marua Abu Risha performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Asghar Ali and Fiete Haack performed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Puntita Siengdee and Siriluck Ponsuksili conceived and designed the experiments, performed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Nares Trakooljul and Dirk Dannenberger analyzed the data, authored or reviewed drafts of the paper, and approved the final draft.

Klaus Wimmers conceived and designed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Data Availability

The following information was supplied regarding data availability:

Raw data are available as Supplemental Files.

Funding

This research was funded by the Doctoral Project Competition of the Leibniz Institute for Farm Animal Biology (FBN). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

10 Citations 2,274 Views 309 Downloads

Your institution may have Open Access funds available for qualifying authors. See if you qualify

Publish for free

Comment on Articles or Preprints and we'll waive your author fee
Learn more

Five new journals in Chemistry

Free to publish • Peer-reviewed • From PeerJ
Find out more