A novel one-step quick assay for detection of SARS-COV2 antibodies across mammalian species

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Biochemistry, Biophysics and Molecular Biology

Main article text

 

Introduction

Materials and Methods

Production of scN-GFP in Escherichia coli

One-step test

Human serum samples

Results

Discussion

Supplemental Information

Supplemental material.

50 pre-pandemic human serum samples were purchased from San Diego Blood Bank in July, 2019. Sample IDs were renamed to protect identifiable information. 40 SARS-CoV2 patient serum samples were purchased from RayBiotech. Sample IDs were renamed and age ranges were provided to protect patient identifiable information. All of the patients were diagnosed by PCR except one with antigen diagnosis. There are 5 PCR negative patients, but they appear to have antibodies against SARS-CoV2 RBD. The only negative sample by the one-step test is B14 that was PCR negative and has low levels of antibodies against SARS-CoV2 RBD.

DOI: 10.7717/peerj.11381/supp-1

40 SARS-CoV2 patient sera purchased from RayBiotech.

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50 pre-pandemic human blood serum purchased from San Diego Blood Bank.

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Raw data from sera of individuals: One-Step tests of pre-pandemic controls (SDBB5 to SDBB15) from San Diego Blood Bank.

The positive control is rabbit anti-scN antibodies diluted in human serum.

DOI: 10.7717/peerj.11381/supp-4

Raw data from sera of individuals: One-Step tests of pre-pandemic controls (SDBB16 to SDBB26) from San Diego Blood Bank.

The positive control is rabbit anti-scN antibodies diluted in human serum.

DOI: 10.7717/peerj.11381/supp-5

Raw data from sera of individuals: One-Step tests of pre-pandemic controls (SDBB27 to SDBB37) from San Diego Blood Bank.

The positive control is rabbit anti-scN antibodies diluted in human serum.

DOI: 10.7717/peerj.11381/supp-6

Raw data from sera of individuals: One-Step tests of pre-pandemic controls (SDBB38 to SDBB48) from San Diego Blood Bank.

The positive control is rabbit anti-scN antibodies diluted in human serum.

DOI: 10.7717/peerj.11381/supp-7

Raw data from sera of individuals: One-Step tests of pre-pandemic controls (SDBB49 to SDBB50) from San Diego Blood Bank.

The positive control is rabbit anti-scN antibodies diluted in human serum.

DOI: 10.7717/peerj.11381/supp-8

Figure S6. One-Step tests of SARS-CoV2 patients (A6-10, B6-10) from RayBiotech.

Raw data from sera of patients A6-10, B6-10 from RayBiotech. The positive control is rabbit anti-scN antibodies diluted in human serum. The negative control is pre-pandemic serum from San Diego Blood Bank.

DOI: 10.7717/peerj.11381/supp-9

Raw data from sera of patients: One-Step tests of SARS-CoV2 patients (A11-15, B11-15) from RayBiotech.

The positive control is rabbit anti-scN antibodies diluted in human serum. The negative control is pre-pandemic serum from San Diego Blood Bank.

DOI: 10.7717/peerj.11381/supp-10

Raw data from sera of patients: One-Step tests of SARS-CoV2 patients (A16-20, B16-20) from RayBiotech.

The positive control is rabbit anti-scN antibodies diluted in human serum. The negative control is pre-pandemic serum from San Diego Blood Bank.

DOI: 10.7717/peerj.11381/supp-11

Additional Information and Declarations

Competing Interests

Xianjin Zhou is the inventor on a provisional patent filing by the University of California San Diego.

Author Contributions

Xianjin Zhou conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Human Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

The studies were approved by Human Research Protections Program at University of California San Diego.

Patent Disclosures

The following patent dependencies were disclosed by the authors:

Methods for Detecting Antibodies (UCSD Ref. No. SD2021-104, Oct 10, 2020)

Data Availability

The following information was supplied regarding data availability:

The raw data are available in the Supplemental Files.

Funding

The study is supported by R21 MH123705 (Xianjin Zhou). There was no additional external funding received for this study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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