DNA methylome and transcriptome analysis established a model of four differentially methylated positions (DMPs) as a diagnostic marker in esophageal adenocarcinoma early detection

View article
Loading...
Bioinformatics and Genomics

Main article text

 

Introduction

Materials and Methods

Database search and data download and initial analysis

Analysis of differential DNA methylation CpGs vs. differentially expressed genes

Data correlational analysis and construction of a candidate diagnostic DNA methylation model

Validation of the diagnostic model of the four DMPs in EAC

Statistical analysis

Results

Identification of DMPs in EAC

Association of DMPs with expression of the corresponding genes

Establishment of DMPs as a diagnostic model in EAC

Validation of this four DMPS as a signature to diagnose EAC

Discussion

Conclusion

Supplemental Information

Raw data: Clinical information.

DOI: 10.7717/peerj.11355/supp-1

Raw data: EAC methylation data.

DOI: 10.7717/peerj.11355/supp-2

Raw data: SQ methylation data.

DOI: 10.7717/peerj.11355/supp-3

Functional enrichment analysis of aberrantly methylated genes.

(A) GO analysis of aberrantly methylated genes. (B) KEGG pathway analysis of aberrantly methylated genes.

DOI: 10.7717/peerj.11355/supp-4

Kaplan Meier curves for four genes.

Kaplan Meier curves of (A) EFS (B) HOXA7 (C) IKZF1 (D) TSHZ3 for overall survival rates based on TCGA EAC samples.

DOI: 10.7717/peerj.11355/supp-5

Correlation between known mediators of the CpG methylation and four genes.

Correlation between known mediators of the CpG methylation and four genes

DOI: 10.7717/peerj.11355/supp-6

Evaluation power of the diagnostic model.

(A) Risk score of EAC tumor and normal tissues in GSE72872. (B) ROC curve of the diagnostic model in GSE72872.

DOI: 10.7717/peerj.11355/supp-7

Examining methylation levels of the four CpGs in external early stage EAC dataset.

Methylation levels of the four CpGs between EAC tumor and normal tissues in GSE89181.

DOI: 10.7717/peerj.11355/supp-8

Additional Information and Declarations

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Weilin Peng conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Guangxu Tu analyzed the data, prepared figures and/or tables, and approved the final draft.

Zhenyu Zhao analyzed the data, prepared figures and/or tables, and approved the final draft.

Boxue He analyzed the data, prepared figures and/or tables, and approved the final draft.

Qidong Cai performed the experiments, analyzed the data, prepared figures and/or tables, and approved the final draft.

Pengfei Zhang analyzed the data, prepared figures and/or tables, and approved the final draft.

Xiong Peng analyzed the data, prepared figures and/or tables, and approved the final draft.

Shuai Shi analyzed the data, prepared figures and/or tables, and approved the final draft.

Xiang Wang conceived and designed the experiments, prepared figures and/or tables, and approved the final draft.

Data Availability

The following information was supplied regarding data availability:

DNA methylation data (Methylation450K) of ESCA, ACC, BLCA, BRCA, CESC, CHOL, COAD, DLBC, GBM, HNSC, KICH, KIRC, KIRP, LAML, LGG, LIHC, LUAD, LUSC, MESO, OV, PAAD, PCPG, PRAD, READ, STAD and UCEC were collected from the UCSC Xena database (https://xenabrowser.net/datapages/).

GSE69270, GSE72872, GSE89181, GSE81334 and GSE104707 datasets are available from GEO.

Funding

This work was supported by the National Natural Science Foundation of China (81672308, 81101767 and 81972195), the Hunan Provincial Key Area R&D Programmes (2019SK2253), and the Scientific Research Program of Hunan Provincial Health Commission (20201047). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

2 Citations 1,956 Views 382 Downloads

Your institution may have Open Access funds available for qualifying authors. See if you qualify

Publish for free

Comment on Articles or Preprints and we'll waive your author fee
Learn more

Five new journals in Chemistry

Free to publish • Peer-reviewed • From PeerJ
Find out more