Luzindole and 4P-PDOT block the effect of melatonin on bovine granulosa cell apoptosis and cell cycle depending on its concentration

View article
Biochemistry, Biophysics and Molecular Biology

Main article text

 

Introduction

Materials and Methods

Bovine granulosa cells isolation and culture

RNA extraction

Real-time PCR

Western blot Analysis

Experimental design

Statistical analysis

Results

Effects of melatonin and melatonin receptor antagonist supplementation on the cell cycle related genes

Effects of melatonin and melatonin receptor antagonist supplementation on granulosa cells apoptosisrelated genes

Effects of melatonin and melatonin receptor antagonist supplementation on granulosa cells antioxidant related genes expression

Discussion

Conclusion

Supplemental Information

Effects of high dose(10−5 M) melatonin and melatonin receptor antagonist supplementation on related genes expression.

Raw data for Ct value of 10 target genes and 1 reference gene, cell cycle, cell apoptosis and antioxidant related genes in four experimental group: control, melatonin group, melatonin plus 4P-PDOT group and melatonin plus luzindole group.

DOI: 10.7717/peerj.10627/supp-1

Effects of low dose(10−9 M) melatonin and melatonin receptor antagonist supplementation on granulosa cells related genes expression.

Raw data for Ct value of 10 target genes and 1 reference gene, cell cycle, cell apoptosis and antioxidant related genes in four experimental group: control, melatonin group, melatonin plus 4P-PDOT group and melatonin plus luzindole group.

DOI: 10.7717/peerj.10627/supp-2

Bcl2, Bax, Caspase-3 and Actin in the control, melatonin group, melatonin plus 4P-PDOT group and melatonin plus luzindole group. Detected by the Western blot.

DOI: 10.7717/peerj.10627/supp-3

Additional Information and Declarations

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Shujuan Wang conceived and designed the experiments, performed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Wenju Liu conceived and designed the experiments, performed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Aiyou Wen analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Bing Yang analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Xunsheng Pang analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Data Availability

The following information was supplied regarding data availability:

Raw data are available in Supplemental Files.

Funding

This study was financially supported by the National Natural Science Foundation (31301972), the Natural Science Foundation of Anhui Province (2008085MC94), the Project Supported by the Plan of Anhui Province for Outstanding Youth Talent in University (gxyq2018049), the Key research and development project of Chuzhou (2018ZN014) and the Key Research and development program of Anhui Province (202004f06020048). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

13 Citations 1,807 Views 294 Downloads

Your institution may have Open Access funds available for qualifying authors. See if you qualify

Publish for free

Comment on Articles or Preprints and we'll waive your author fee
Learn more

Five new journals in Chemistry

Free to publish • Peer-reviewed • From PeerJ
Find out more