Dysregulated levels of glycogen synthase kinase-3β (GSK-3β) and miR-135 in peripheral blood samples of cases with nephrotic syndrome

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Biochemistry, Biophysics and Molecular Biology

Main article text

 

Introduction

Materials & Methods

Study subjects

RNA extraction and evaluation in PBMCs

Statistical analysis

Results

Discussion

Conclusions

Supplemental Information

ROC curve analysis of miR-135 in plasma and PBMCs.

DOI: 10.7717/peerj.10377/supp-1

Real-time PCR (Ct values) generated by micPCR (version 2.6.5) used in analysis and preparation of Figs. 13 and Tables 2 and 3

DOI: 10.7717/peerj.10377/supp-2

Additional Information and Declarations

Competing Interests

The authors declare there are no competing interests.

Author Contributions

Mohammadreza Ardalan conceived and designed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Seyyedeh Mina Hejazian performed the experiments, analyzed the data, authored or reviewed drafts of the paper, sampling, and approved the final draft.

Hassan Sharabiyani and Elham Ahmadian performed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Farahnoosh Farnood performed the experiments, analyzed the data, authored or reviewed drafts of the paper, and approved the final draft.

Amirhossein Ghafari Aghdam performed the experiments, authored or reviewed drafts of the paper, sampling, and approved the final draft.

Milad Bastami performed the experiments, prepared figures and/or tables, designed the primers and did revise the manuscript, and approved the final draft.

Sepideh Zununi Vahed conceived and designed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Magali Cucchiarini analyzed the data, authored or reviewed drafts of the paper, and approved the final draft.

Human Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

Tabriz University of Medical Sciencesgranted Ethical approval to carry out the study within its facilities (Ethical Application Ref: IR.TBZMED.REC.1397.1021).

Data Availability

The following information was supplied regarding data availability:

Real-time data of miR-135 & GSK-2B are available in a Supplemental File.

Funding

This work was financially supported by the Kidney Research Center, Tabriz University of Medical Sciences and a Research Grant (Grant No. 62640). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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