Exploration of ovine milk whey proteome during postnatal development using an iTRAQ approach

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Biochemistry, Biophysics and Molecular Biology

Main article text

 

Introduction

Materials & Methods

Ethics statement

Sample collection and whey protein preparing

Protein digestion and iTRAQ labeling

Peptide fractionation with strong cation exchange chromatography

Liquid chromatography (LC)-electrospray ionization (ESI) tandem MS (MS/MS) analysis by Q Exactive

Sequence database searching and data analysis

Bioinformatic analysis

Results

Identification of differential proteins in the whey fractions of Hu sheep after delivery

Gene ontology analysis of the DEPs

KEGG pathway analysis of the identified proteins

Cluster analysis of the differentially expressed whey proteins

Discussion

Conclusions

Supplemental Information

The results of protein identification and relative expression abundance by matching to ovis aries proteomics database

DOI: 10.7717/peerj.10105/supp-1

The results of peptide identification and relative expression abundance by matching to ovis aries proteomics database

DOI: 10.7717/peerj.10105/supp-2

The results of protein identification and relative expression abundance by matching to cetartiodactyla proteomics database

DOI: 10.7717/peerj.10105/supp-3

The results of peptide identification and relative expression abundance by matching to cetartiodactyla proteomics database

DOI: 10.7717/peerj.10105/supp-4

Proteins identified at different lactation stages

DOI: 10.7717/peerj.10105/supp-5

The 121 kinds of DEPs at different lactation stages

DOI: 10.7717/peerj.10105/supp-6

Additional Information and Declarations

Competing Interests

The authors declare there are no competing interests.

Author Contributions

Xueying Zhang performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Fadi Li conceived and designed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Fang Qin performed the experiments, prepared figures and/or tables, and approved the final draft.

Wanhong Li analyzed the data, authored or reviewed drafts of the paper, and approved the final draft.

Xiangpeng Yue conceived and designed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

The experimental procedures were approved by the Ethics Committee of College of Pastoral Agriculture Science and Technology of Lanzhou University.

Data Availability

The following information was supplied regarding data availability:

The raw measurements are available in the Supplementary Files.

Funding

This research was supported by the National Key Technology Research and Development Program of the Ministry of Science and Technology of China (2015BAD03B05), the China Agriculture Research System (CARS-38), and the Program for Changjiang Scholars and Innovative Research Team in University (IRT13019). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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