Calcium affinity of human α-actinin1
Department of Chemistry, Umeå University, Umeå, Sweden
- Published
- Accepted
- Subject Areas
- Biochemistry, Biophysics
- Keywords
- EF-hand, α-actinin, calcium binding
- Copyright
- © 2015 Backman
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ PrePrints) and either DOI or URL of the article must be cited.
- Cite this article
- 2015. Calcium affinity of human α-actinin1. PeerJ PrePrints 3:e988v1 https://doi.org/10.7287/peerj.preprints.988v1
Abstract
Due to alternative splicing the human ACTN1 gene codes for three different transcripts of α‑actinin; one isoform that is expressed only in the brain and two with a more general expression pattern. The sequence difference is located to the C-terminal domains and the EF-hand motifs. Therefore any functional or structural distinction should involve this part of the protein. To investigate this further, the calcium affinities of these three isoforms of α‑actinin1 have been determined by isothermal calorimetry.
Author Comment
This is a submission to PeerJ for review.