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Supplemental Information

Supplementary data for the manuscript: Trans-species Polymorphism at Antimicrobial Innate Immunity Cathelicidin Genes of Atlantic cod and Related Species

DOI: 10.7287/peerj.preprints.924v1/supp-1

Additional Information

Competing Interests

The authors declare there are no competing interests.

Author Contributions

Katrín Halldórsdóttir conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Einar Árnason conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

The Icelandic Committee for Welfare of Experimental Animals, Chief Veterinary Office at the Ministry of Agriculture, Reykjavik, Iceland has determined that the research conducted here is not subject to the laws concerning the Welfare of Experimental

Animals (The Icelandic Law on Animal Protection, Law 15/1994, last updated with Law 157/2012). DNA was isolated from tissue taken from dead fish on board research vessels. Fish were collected during the yearly surveys of the Icelandic Marine Research Institute. All research plans and sampling of fish, including the ones for the current project, have been evaluated and approved by the Marine Research Institute Board of Directors. The Board comprises the Director General, Deputy Directors for Science and Finance and heads of the Marine Environment Section, the Marine Resources Section, and the Fisheries Advisory Section. Samples were also obtained from dead fish from marine research institutes in Norway, the Netherlands, Canada and the US that were similarly approved by the respective ethics boards. The samples from the US used in this study have been described in Cunningham et al. (2009) and the samples from Norway in Árnason and Pálsson (1996). The samples from Canada consisted of DNA isolated from the samples described in Pogson (2001). The samples from the Netherlands were obtained from the Beam-Trawl-Survey (http://www.wageningenur.nl/en/Expertise-Services/

Research-Institutes/imares/Weblogs/Beam-Trawl-Survey.htm) of the Institute for Marine Resources & Ecosystem Studies (IMARES), Wageningen University, the Netherlands, which is approved by the IMARES Animal Care Committee and IMARES Board of Directors.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

New sequences generated in this study have GenBank accession numbers KJ831349 – KJ831391

Funding

Funding was provided by Icelandic Science Foundation grant of excellence (nr. 40303011), a University of Iceland Research Fund grant, a Svala Árnadóttir private foundation grant to Einar Árnason and a doctoral grant from the University of Iceland Research Fund to Katrín Halldórsdóttir. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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