Figure S1. Sequence of an amber-inserted EGFP expression construct
The amber-inserted EGFP expression sequence was cloned into pDONR221 using a standard Gateway reaction by BP recombination.
Fig. S2. EGFP fluorescence of bacteria carrying the ΔMJR1 negative control plasmid
A bacterial strain carrying both the ΔMJR1 and the amber-inserted EGFP expression plasmid was evaluated. EGFP fluorescence was measured at various IY concentrations. Data are shown as mean SEM. n = 3 independent experiments. Statistical analysis was performed using Welch’s t-test (α = 0.05). No significant differences were detected in EGFP fluorescence intensity.
Fig. S3. Growth curves at an extremely high IY concentration
(A) pTYR MjIYRS2-1(D286) MJR13 and the amber-inserted EGFP expression plasmid. (B) pTYR MjIYRS2-1(D286) MJR13 alone. (C) The parental strain BL21-AI without any plasmids. Open circle, 0 M; filled circle, 3 10-3 M.