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Supplemental Information

Figure S1. Sequence of an amber-inserted EGFP expression construct

The amber-inserted EGFP expression sequence was cloned into pDONR221 using a standard Gateway reaction by BP recombination.

DOI: 10.7287/peerj.preprints.855v1/supp-1

Fig. S2. EGFP fluorescence of bacteria carrying the ΔMJR1 negative control plasmid

A bacterial strain carrying both the ΔMJR1 and the amber-inserted EGFP expression plasmid was evaluated. EGFP fluorescence was measured at various IY concentrations. Data are shown as mean  SEM. n = 3 independent experiments. Statistical analysis was performed using Welch’s t-test (α = 0.05). No significant differences were detected in EGFP fluorescence intensity.

DOI: 10.7287/peerj.preprints.855v1/supp-2

Fig. S3. Growth curves at an extremely high IY concentration

(A) pTYR MjIYRS2-1(D286) MJR13 and the amber-inserted EGFP expression plasmid. (B) pTYR MjIYRS2-1(D286) MJR13 alone. (C) The parental strain BL21-AI without any plasmids. Open circle, 0 M; filled circle, 3  10-3 M.

DOI: 10.7287/peerj.preprints.855v1/supp-3

Data for Figure 3 (histogram)

DOI: 10.7287/peerj.preprints.855v1/supp-6

Additional Information

Competing Interests

The author declares they have no competing interests.

Author Contributions

Yusuke Kato conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.


This work was supported by JSPS KAKENHI Grant number 25660281. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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