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Supplemental Information

Tukey boxplot of ancestral Ne from msvar analyses

Estimates are given on the log10 scale. Datasets A and B represent different subsamples of the full dataset from each respective year.

DOI: 10.7287/peerj.preprints.788v1/supp-1

Tukey boxplot of the time of population size change from msvar analyses

Estimates are given on the log10 scale. Datasets A and B represent different subsamples of the full dataset from each respective year.

DOI: 10.7287/peerj.preprints.788v1/supp-2

Collection information for samples included in our study

Samples included in dataset 1 are listed first, sorted by population (in the same order as Table 1), followed by samples included in datasets 2 and 3 sorted by state and county. Note that samples included in dataset 1 may have also been included in dataset 2 if they were collected in 2002. Genbank accession numbers are provided for all unique sequences for each marker.

DOI: 10.7287/peerj.preprints.788v1/supp-3

Locus information for IMa2 analysis

DOI: 10.7287/peerj.preprints.788v1/supp-4

Additional Information

Competing Interests

The authors declare they have no competing interests.

Author Contributions

Maarten Vonhof conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Amy L. Russell conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

The vast majority of samples were collected by other researchers as part of their biodiversity studies, and these researchers had to obtain appropriate permits and IACUC approval for their own research. Three samples were collected by one of the authors (MJV) under Institutional Animal Care and Use Committee approval (protocol 05-03-01).

Field Study Permissions

The following information was supplied relating to field study approvals (i.e., approving body and any reference numbers):

Three of the samples collected by one author (MJV) were collected with permission from the Michigan Department of Natural Resources (permit SC1257).

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

GenBank - accession numbers will be provided prior to publication

Funding

We received funding from U.S. Department of Energy, 20% Wind by 2030 Program (grant DE-EE0000533). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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