Prospecting Russula senecis: A delicacy among the tribes of West Bengal
- Published
- Accepted
- Subject Areas
- Evolutionary Studies, Mycology, Taxonomy, Pharmacology
- Keywords
- Antimicrobial property, Antioxidant activity, Edible mushroom, HPLC, Internal Transcribed Spacer sequence, Molecular phylogeny, Taxonomy
- Copyright
- © 2015 Khatua et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ PrePrints) and either DOI or URL of the article must be cited.
- Cite this article
- 2015. Prospecting Russula senecis: A delicacy among the tribes of West Bengal. PeerJ PrePrints 3:e707v2 https://doi.org/10.7287/peerj.preprints.707v2
Abstract
Russula senecis, a worldwide distributed mushroom, is exclusively popular among the tribal communities of West Bengal for food purposes. The present study focuses on its reliable taxonomic identification through macro-, micro-morphological features and DNA barcoding, confirmation of its systematic placement by phylogenetic analyses, myco-chemicals and functional activities. For the first time, complete Internal Transcribed Spacer region of R. senecis has been sequenced and its taxonomic position within subsection Foetentinae under series Ingratae of the subgen. Ingratula is confirmed through phylogenetic analysis. For exploration of its medicinal properties, dried basidiocarps were subjected for preparation of a heat stable phenol rich extract (RusePre) using water and ethanol as solvent system. The antioxidant activity was evaluated through hydroxyl radical scavenging (EC50 5 µg/ml), chelating ability of ferrous ion (EC50 0.158 mg/ml), DPPH radical scavenging (EC50 1.34 mg/ml), reducing power (EC50 2.495 mg/ml) and total antioxidant activity methods (13.44 µg ascorbic acid equivalent/mg of extract). RusePre exhibited antimicrobial potentiality against Listeria monocytogenes, Bacillus subtilis, Pseudomonas aeruginosa and Staphylococcus aureus. Furthermore, different parameters were tested to investigate its chemical composition which revealed the presence of appreciable quantity of phenolic compounds along with carotenoids and ascorbic acid. HPLC-UV fingerprint indicated probable existence of at least 13 phenolics of which 10 were identified (pyrogallol> kaempferol> quercetin> chlorogenic acid> ferulic acid, cinnamic acid> vanillic acid> salicylic acid> p-coumaric acid> gallic acid). Result from the present work suggests that the fraction, RusePre, may open novel prospect as a functional ingredient in antioxidant supplements and in drugs to treat infectious disease.
Author Comment
This is a revised version to PeerJ for review.