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Additional Information

Competing Interests

AM, CA and NC have a patent on the PNA-FISH probe described in this paper (Portuguese patente office (INPI20121000090117). NC is an Investigador FCT (Portuguese national funds).

Author Contributions

António Machado performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Joana Castro performed the experiments, reviewed drafts of the paper.

Tatiana Cereija performed the experiments, analyzed the data, reviewed drafts of the paper.

Carina Almeida analyzed the data, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Nuno Cerca conceived and designed the experiments, wrote the paper, reviewed drafts of the paper.

Human Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

Subcomissão de Ética para as Ciências da Vida e Saúde, from Universidade do Minho (process SECVS 003/2013)

Funding

This work was supported by European Union funds (FEDER/COMPETE) and by national funds (FCT) under the project FCOMP-01-0124-FEDER-008991 (PTDC/BIA-MIC/098228/2008). AM was funded by the FCT individual fellowship SFRH/BD/62375/2009. Other fundings came from the FCT Strategic Project PEst-OE/EQB/LA0023/2013, the Project NORTE-07-0124-FEDER-000027, co-funded by the Programa Operacional Regional do Norte (ON.2 – O Novo Norte), QREN, FEDER, and the project FCOMP-01-0124-FEDER-008991 (RECI/BBB-EBI/0179/2012). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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