Evaluation of autotrophic and mixotrophic regimen Chlorella pyrenoidosa cells in various wastes water for its biochemical composition and biomass production
- Published
- Accepted
- Subject Areas
- Biotechnology, Microbiology
- Keywords
- C. pyrenoidosa, wastewater, autotrophic, mixotrophic, biomass, biochemical composition.
- Copyright
- © 2014 Dhull et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ PrePrints) and either DOI or URL of the article must be cited.
- Cite this article
- 2014. Evaluation of autotrophic and mixotrophic regimen Chlorella pyrenoidosa cells in various wastes water for its biochemical composition and biomass production. PeerJ PrePrints 2:e681v1 https://doi.org/10.7287/peerj.preprints.681v1
Abstract
The present study investigates the possibility of integrating an existing industrial large scale biomass production with the treatment of waste water in which a mixture of organic and inorganic rich pollutants was used as a medium. This study suggests that the replacement of a defined medium with a complete mixotrophic medium gives a significant statistical difference in terms of growth parameters i.e. biomass production and specific growth rate. The green microalga C. pyrenoidosa was cultivated under different mixotrophic conditions for evaluation of biomass production. Inorganic defined fog’s medium supplemented, with raw dairy wastewater led to 1.37g/L biomass production in comparison to 1.2g/L obtained with pure glucose revealing 14.16% increase. The study also involves the supplementation of raw dairy wastewater as an organic carbon source in an inorganic medium comprising municipal treated water and reverse osmosis (RO) treated wastewater and attained 2.4g/L and 1.6g/L of biomass respectively, as compared to 0.3g/L and 0.16g/L obtained in the wastewaters alone revealing 700% and 900% increase respectively. Mixotrophic regimen cells as analyzed by a 2D Fourier transform infrared (FTIR) spectroscopy for its biochemical content revealed that fog’s blended raw dairy waste (RDW) regimen cells had maximum Carbohydrate/Amide ratio. The study suggests that the mixotrophic regimen C. pyrenoidosa cells can show appropriate growth in a mixture of waste waters and the same comes out to be a cost effective and feasible alternative commercial medium for biomass production without requiring any expensive organic carbon sources in the culture medium.
Author Comment
The present study has been carried out to evaluate and compare autotrophic and mixotrophic growth of C. pyrenoidosa, by utilizing a raw dairy waste as organic medium combined with various inorganic rich wastewater media as a cultural medium.