Urinary nucleic acids can be concentrated, dried on membranes and stored at room temperature in vacuum bags
- Published
- Accepted
- Subject Areas
- Biochemistry, Molecular Biology
- Keywords
- urinary nucleic acid, preservation, concentration, nylon membrane, room temperature
- Copyright
- © 2014 Zhang et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ PrePrints) and either DOI or URL of the article must be cited.
- Cite this article
- 2014. Urinary nucleic acids can be concentrated, dried on membranes and stored at room temperature in vacuum bags. PeerJ PrePrints 2:e566v1 https://doi.org/10.7287/peerj.preprints.566v1
Abstract
Urine accumulates traces of changes that occur in the body and can potentially serve as a better biomarker source. Urinary nucleic acids are a promising class of non-invasive disease biomarkers. However, long-term frozen human urine samples are not a good source for the extraction of nucleic acids. In this paper, we demonstrate that urinary nucleic acids can be concentrated, dried on membranes and stored in vacuum bags at room temperature for several months. The amount of total RNA on the membranes after storage at room temperature for three months was unchanged. The levels of miR-16 and miR-21 exhibited no significant differences (P = 0.564, 0.386). This simple and economical method makes the large-scale storage of clinical samples of urinary nucleic acids possible.
Author Comment
This is a submission to PeerJ PrePrints for comments.