Media formulations in phenotype assay plates
Supplemental Table 1: In-house phenotypic array plate. Each stock well contains 1ml of 6X basal media (6X MOPS media, 57 mM NH4Cl, 1.5 mM NaSO4, 30 μM CaCl2, 6 mM MgSO4, 1.9M NaCl, 7.92 mM K2HPO4, 60 mM KCl, 36 μM FeCl3) and 1ml of 5X carbon substrate. The substrates are used at a final concentration of 0.2% unless specified.
Orthologous groups in all the genomes
Supplemental Table 2. Orthologous groups identified in all the genomes. OG is a unique identifier; Function is the most common function annotated for all the proteins; The remaining columns identify the protein encoding genes (pegs) found in each genome or None if the gene is not present.
2013 Southern Line Islands Research Expedition
Supplemental Figure 1: Expedition route of the 2013 Southern Line Island Expedition. The expedition left Papeete Harbor and circumnavigated the Southern Line Islands in a clockwise direction.
Sampling the reefs
Supplemental Figure 2: Examples of sampling surface for bacterial isolates. VRT35 and VRT38 were isolated from coral surfaces, while VRT37 and VRT41 were isolated from algae surfaces.
Number of bases sequenced and number of contigs generated
Supplemental Figure 3. Number of bases sequenced and number of contigs generated. The best-fit power regression of the data (shown with a gray line) has the equation [number of contigs] = 3.87 * 1010 [base piars sequenced]-0.95 with an R2 of 0.667.
Growth curves in 48 different media showing growth in some wells
Supplemental Figure 4: Growth curve of bacterial isolates in 48 different carbon sources indicated in Supplementary Table 1.
Heat map showing growth characteristics of all the isolates
Supplemental Figure 5: Heatmap showing the growth characteristics of each bacterial isolates from the 2013 Southern Line Island expedition in 48 different carbon sources.
Negative controls don't grow
Supplemental Figure 6: Growth curve of negative controls and filtered seawater-only samples in 48 different carbon sources indicated in Supplementary Table 1.