Deep eutectic solvents aqueous two-phase system based ultrasonically assisted extraction of ursolic acid (UA) from Cynomorium songaricum Rupr.
- Published
- Accepted
- Subject Areas
- Biochemistry, Bioengineering, Food Science and Technology, Plant Science
- Keywords
- Choline chloride-Glucose, Ursolic acid, Aqueous two-phase systems, Conventional UAE, Response surface methodology
- Copyright
- © 2017 Zhang et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
- Cite this article
- 2017. Deep eutectic solvents aqueous two-phase system based ultrasonically assisted extraction of ursolic acid (UA) from Cynomorium songaricum Rupr. PeerJ Preprints 5:e3495v1 https://doi.org/10.7287/peerj.preprints.3495v1
Abstract
Deep eutectic solvents (DESs) are new green solvents that have attracted the attention of the scientific community mainly due to their unique properties and special characteristics, which are different from those of traditional solvents.A method based on ultrasonically assisted deep eutectic solvent aqueous two-phase systems( UAE-DES-ATPS) was developed for extracting ursolic acid (UA) from Cynomorium songaricum Rupr. Four different types of choline chloride-based DESs were prepared.Choline chloride-glucose (ChCl-Glu) exhibited good selective extraction ability. An optimum DES-ATPS of 36% (w/w) ChCl-Glu and 25% (w/w) K2HPO4 was considered to be a satisfactory system for extracting UA. Response surface methodology (RSM) method was used to optimize the extraction of UA using UAE-DES-ATPS. The optimum ultrasound-assisted conditions were as follows: solvent to solid ratio of 15:1 (g/g), ultrasound power of 470 W, and extraction time of 54 min. Compared with the conventional UAE method, the yields were basically the same, but the presented method had higher purity. The structure of UA did not change between pure UA and UA in the upper phase by UV–vis and FT-IR. This approach using ChCl-based DES-ATPS as a novel extraction system and ultrasound as a source of energy provided better choice for the separation of active components from other natural products.
Author Comment
There is no literature reported to optimize the extraction conditions for UA using ultrasonically assisted deep eutectic solvent aqueous two-phase systems. Thus, the main aim of the present work was to establish a novel extraction method. The data presented in this article demonstrate the feasibility of the extraction of UA by ultrasonically assisted deep eutectic solvent aqueous two-phase systems, which was an efficient method compared to conventional ultrasonic assisted extraction.