Complete mitochondrial genome sequence of the “copper moss” Mielichhoferia elongata reveals independent nad7 gene functionality loss
- Published
- Accepted
- Subject Areas
- Biodiversity, Evolutionary Studies, Genomics, Plant Science, Taxonomy
- Keywords
- Mitochondrial genome, Bryophyta, phylogenetic tree, “copper moss” Mielichhoferia elongate, nad7, simple sequence repeats, Phylogenetic tree, Simple sequence repeats, “Copper moss” Mielichhoferia elongata
- Copyright
- © 2017 Goruynov et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
- Cite this article
- 2017. Complete mitochondrial genome sequence of the “copper moss” Mielichhoferia elongata reveals independent nad7 gene functionality loss. PeerJ Preprints 5:e3465v1 https://doi.org/10.7287/peerj.preprints.3465v1
Abstract
The mitochondrial genome of moss Mielichhoferia elongata has been sequenced and assembled with Spades genome assembler. It consists of 100,342 base pairs and has practically the same gene set and its order as in other known bryophyte chondriomes. The genome contains 66 genes including three rRNAs, 24 tRNAs, and 40 conserved mitochondrial proteins genes. Unlike the majority of previously sequenced bryophyte mitogenomes, it lacks the functional nad7 gene. The phylogenetic reconstruction and scrutiny analysis of the primary structure of nad7 gene carried out in this study suggest its independent pseudogenization in different bryophyte lineages. Evaluation of the microsatellite (simple sequence repeat) content of the Mielichhoferia elongata mitochondrial genome indicates that it could be used as a tool in further studies as a phylogenetic marker. The strongly supported phylogenetic tree presented here, derived from 31 protein coding sequences of 40 bryophyte species is consistent with other reconstructions based on a number of different data sets.
Author Comment
This is a submission to PeerJ for review.
Supplemental Information
Mitogenome map of M. elongata (MF417767)
The phylogenetic tree based on nuclear rDNA region (5.8S rDNA - ITS 2 - 5‘-end of 26S rDNA). The alignment consists of 440 positions. The tree reconstruction was conducted in TREECON software (Van de Peer & De Wachter, 1994) using the Neighbor-Joining method (Saitou & Nei 1987) with 500 bootstrap replications. Bootstrap support values >50% are shown next to the branches. The evolutionary distances were computed using the Kimura method (1980) with gaps taken into account as it implemented in the TREECON package.