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Arbutin increases Caenorhabditis elegans longevity and stress resistance

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8 days ago
RT @fruitWormPapers: Arbutin increases Caenorhabditis elegans longevity and stress resistance https://t.co/gXh3LkKbye
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Arbutin increases Caenorhabditis elegans longevity and stress resistance https://t.co/UgxOtlGlhx
Arbutin increases Caenorhabditis elegans longevity and stress resistance https://t.co/gXh3LkKbye
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Supplemental Information

Effect of arbutin on the lifespan of C. elegans

Wild type animals (N = 120-182 in each group) were treated without (0 mM) or with low (0.5, 2.5 mM), moderate (5 mM, p < 0.001) and high (10, 20 mM) doses of arbutin at 20 ℃ from birth, when survival was monitored. The experiment was repeated multiple times and a representative trial is shown.

DOI: 10.7287/peerj.preprints.3405v1/supp-1

Effect of pretreatment with arbutin on resistance to stress in C. elegans

Animals were treated with 5 mM arbutin from birth to 120 hours at 20 ℃ and exposed to a variety of stressors. Worms pretreated with arbutin survived significantly longer after (A-B) 35 ℃ heat shock (N = 66-84 animals, p < 0.05), (C-D) exposure to 200 μM juglone (N = 90 animals, p < 0.001) or (E-F) UV irradiation at 1000 J/m2 (N = 68-96 animals, p < 0.001). Each experiment is representative of three independent trials.

DOI: 10.7287/peerj.preprints.3405v1/supp-2

Effect of arbutin on ROS accumulation on C. elegans

Wild type N2 animals treated with 5 mM arbutin accumulated less ROS than wild type N2 control animals. (N = 24-25 times and 20 animals per group . p < 0.001).

DOI: 10.7287/peerj.preprints.3405v1/supp-3

Effect of arbutin on resistance to stress in transgenic strain CF1038 C. elegans

Animals were treated with 5 mM arbutin from birth to 120 hours at 20 ℃ and exposed to a variety of stressors. There was no survival significance with worms pretreated with or without arbutin after (A-B) 35 ℃ heat shock (N = 74 animals, p > 0.05), (C-D) exposure to 200 μM juglone (N = 96 animals, p > 0.05) or (E-F) UV irradiation at 1000 J/m2 (N = 85-88 animals, p > 0.05). Each experiment is representative of three independent trials.

DOI: 10.7287/peerj.preprints.3405v1/supp-4

Effect of arbutin on DAF-16::GFP nuclear localization on transgenic strain TJ356

Worms treated with 5 mM arbutin (A-B) displayed a fluorescence intensity that was significantly upregulated (E) relative to the control group (C-D). (N = 10 animals per group, p < 0.001).

DOI: 10.7287/peerj.preprints.3405v1/supp-5

Effect of arbutin on relative mRNA levels of daf-16, sod-3 and hsp-16.2 of C. elegans. )

daf-16 (P < 0.001) and its downstream targets sod-3 (P < 0.05) and hsp-16.2 (P < 0.001) were all upregulated with arbutin treatment relative to the control. (N = 3 and 200 animals per group, p < 0.05).

DOI: 10.7287/peerj.preprints.3405v1/supp-6

Effect of arbutin on brood size of C. elegans

There was no significant difference in the number of total progeny between arbutin-treated and untreated animals (N = 10 animals per group, p > 0.05).

DOI: 10.7287/peerj.preprints.3405v1/supp-7

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Lin Zhou conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Xueqi Fu conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Liyan Jiang conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Lu Wang performed the experiments, reviewed drafts of the paper.

Shuju Bai performed the experiments, reviewed drafts of the paper.

Yan Jiao performed the experiments, reviewed drafts of the paper.

Shu Xing analyzed the data, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Wannan Li analyzed the data, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Junfeng Ma conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Data Deposition

The following information was supplied regarding data availability:

The raw data has been provided in the Supplemental Files.

Funding

This work was supported by grants from the National Natural Science Foundation of China (No. 31000358 for Junfeng Ma and No. 31670795 for Xueqi Fu), Jilin Province Postdoctoral Science Foundation (for Junfeng Ma) and Changbai Mountain Research Support Foundation of 2017 (No. 440050117010 for Xueqi Fu). Moreover, C. elegans strains were provided by the CGC, which was funded by NIH Office of Research Infrastructure Programs (P40 OD010440). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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