Comparative transcriptomic analysis of heterophylly of the aquatic plant Potamogeton octandrus (Potamogetonaceae)
- Published
- Accepted
- Subject Areas
- Cell Biology, Developmental Biology, Evolutionary Studies, Genetics, Plant Science
- Keywords
- Gene expression, Potamogeton octandrus, Transcriptome, Heterophyllous leaves
- Copyright
- © 2017 He et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
- Cite this article
- 2017. Comparative transcriptomic analysis of heterophylly of the aquatic plant Potamogeton octandrus (Potamogetonaceae) PeerJ Preprints 5:e3336v1 https://doi.org/10.7287/peerj.preprints.3336v1
Abstract
Many plant species exhibit heterophylly, displaying different leaves upon a single plant. The molecular mechanisms regulating this phenomenon, however, have remained elusive. In this study, the transcriptomes of submerged and floating leaves of an aquatic heterophyllous plant, Potamogeton octandrus Poir, were sequenced using a high-throughput sequencing technique (RNA-Seq), which aims to assist with the gene discovery and functional studies of genes involved in heterophyllous leaf development. A total of 81,103 unigenes were identified from the submerged and floating leaves, and a total of 6,822 differentially expressed genes (DEGs) were identified by comparing the samples from each developmental stage. KEGG pathway enrichment analysis categorized these unigenes into 128 pathways (p-value < 10-5). A total of 24,025 differentially expressed genes were involved in the carbon metabolic pathway, biosynthesis of amino acids, ribosomes, and plant-pathogen interaction. KEGG pathway enrichment analysis categorized a total of 70 DEGs into plant hormone signal transduction pathways. This study describes the initial results of the high-throughput transcriptome sequencing of heterophylly. Understanding the transcriptomes of floating and submerged leaves of the aquatic plant P. octandrus will assist with gene cloning and functional studies of genes involved in leaf development. This is especially the case with those involved in heterophyllous leaf development.
Author Comment
This is a submission to PeerJ for review.
Supplemental Information
Supplemental information for this article can be found online
Supplemental information for this article can be found online.