Effects of accelerated ageing and cryopreservation on seeds and embryonic axes of Phaseolus vulgaris L and Arachis hypogaea L. Germination and seedlings vigor
Author and article information
Abstract
The effects of artificial aging and cryopreservation methods on the germination of seeds and embryonic axes of Phaseolus vulgaris and Arachis hypogaea , were studied. Aging and cryopreservation treatments in bean seeds not affect the germination percentage, reaching values not significantly different from control. Germination percentage was higher when isolated embryonic axes were studied. Aging treatments gave rise to larger bean seedlings than the controls and cryopreservation treatments affected the size depending on the explant used. In groundnut seeds, aging treatments and cryopreservation positively affected germination and vigor of seedlings, but embryonic axes from not rehydrated aging seeds not germinate. Undried groundnut seeds not survive cryopreservation, while the dried ones showed a germination behavior superior to the control. Embryonic axes tolerate immersion in liquid nitrogen but when droplet-vitrification was used, formed callus instead of seedlings. Electrolyte leakage was always higher in embryonic axes than in seeds, both in beans and groundnut, and not to have a significant effect, under the conditions studied, on germination and vigor of seedlings. The present study evidences the possibility of cryopreserving, by simple procedures, germplasm of beans and groundnut, and to verify the aging tolerance of the seeds and embryonic axes of these important species.
Cite this as
2017. Effects of accelerated ageing and cryopreservation on seeds and embryonic axes of Phaseolus vulgaris L and Arachis hypogaea L. Germination and seedlings vigor. PeerJ Preprints 5:e3203v1 https://doi.org/10.7287/peerj.preprints.3203v1Author comment
This is a submission to PeerJ for review.
Sections
Supplemental Information
Raw data germination and seedlings development GEA
Raw data germination and seedlings development GS
Raw data germination and seedling developmente BEA
Raw data germination and seedlings development BS
Additional Information
Competing Interests
The authors declare that they have no competing interests.
Author Contributions
Marcelo Tacán performed the experiments, analyzed the data, contributed reagents/materials/analysis tools.
César Tapia analyzed the data, contributed reagents/materials/analysis tools, reviewed drafts of the paper.
César Pérez conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.
Funding
This work was sponsored by the PROMETEO Project of the Secretariat of Higher Education, Science, Technology and Innovation (SENESCYT) of the Republic of Ecuador The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
