Visitors   Views   Downloads
NOT PEER-REVIEWED
"PeerJ Preprints" is a venue for early communication or feedback before peer review. Data may be preliminary.

Supplemental Information

Supplementary Figure S1a

(i). The total protein of each cell fraction visualized on a stain free gel cytoplasmic, (C); nucleoplasmic /nuclear soluble, (NS); nuclear chromatin bound pellet, (NCBp). The strong band in the nuclear bound fraction is the micrococcal nuclease added to degrade the associated DNA before SDS-PAGE. (ii). Validation of the antibodies used to detect CITED1 using Duolink/IF and western blots. AB15096 used in figure 1a has been previously validated using siRNA (Howlin et al., 2015) .

DOI: 10.7287/peerj.preprints.3146v1/supp-1

Supplementary Figure S1b

The cell fixation protocol provided by Active Motif

DOI: 10.7287/peerj.preprints.3146v1/supp-2

Supplementary Data S2a

The ordered lists of genes from the ChIP-seq heatmap presented in figure 2a

DOI: 10.7287/peerj.preprints.3146v1/supp-3

Supplementary Data S2b

Summary statistics for the MITF ChIP-seq data analysis

DOI: 10.7287/peerj.preprints.3146v1/supp-4

Supplementary Data S2c

MITF ChIP-seq lists of occupied genes and active regions respectively

DOI: 10.7287/peerj.preprints.3146v1/supp-5

Supplementary Data S3a

Known transcription factor motif identification for siNEG MITF ChIP-seq

DOI: 10.7287/peerj.preprints.3146v1/supp-6

Supplementary Data S3b

De novo transcription factor motif identification for siNEG MITF ChIP-seq

DOI: 10.7287/peerj.preprints.3146v1/supp-7

Supplementary Data S3c

Known transcription factor motif identification for siCITED1 MITF ChIP-seq

DOI: 10.7287/peerj.preprints.3146v1/supp-8

Supplementary Data S3d

De novo transcription factor motif identification for siITED1 MITF ChIP-seq

DOI: 10.7287/peerj.preprints.3146v1/supp-9

Supplementary Data S4a

UCSC tracks BED file for the MITF-ChIP-seq experiment (siNEG and siCITED1)

DOI: 10.7287/peerj.preprints.3146v1/supp-10

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Barbara Lettiero conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, reviewed drafts of the paper.

Martin Lauss analyzed the data, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Ake Borg contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Sofia Gruvberger-Saal contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Goran B Jönsson contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Jillian Howlin conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Data Deposition

The following information was supplied regarding data availability:

All data referred to is included in supplementary files

Funding

This work was supported by This work was supported by Mrs. Berta Kamprad Foundation (JH, SGS, GJ, AB), Swedish Research Council (GJ, AB), Gunnar Nilsson Cancer Foundation (GJ, AB), Swedish Cancer Society (GJ, AB) and The Gustav Vth Jubileefoundation (GJ) and BioCARE (GJ, SGS). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


Add your feedback

Before adding feedback, consider if it can be asked as a question instead, and if so then use the Question tab. Pointing out typos is fine, but authors are encouraged to accept only substantially helpful feedback.

Some Markdown syntax is allowed: _italic_ **bold** ^superscript^ ~subscript~ %%blockquote%% [link text](link URL)
 
By posting this you agree to PeerJ's commenting policies