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Supplemental Information

Raw data for 285 Hungarian draft sequences

DOI: 10.7287/peerj.preprints.3087v1/supp-1

A maximum likelihood tree represents the phylogenetic relationship among 285 partial mtDNA D-loop sequences from members of the genus Equus including haplotypes of the Hungarian draft and reference sequence

The phylogenetic tree was based on the Tamura-Nei model of evolution with gamma distribution of rates and 1,000 bootstrap replicates (Tamura & Nei, 1993). Different colors represent different haplogroups differentiated by sequence motifs of the mtDNA clusters by [25]: A1 (light purple), A2 (dark blue), A5 (brown), A6 (light blue), B1 (yellow), B2 (turquoise), C1 (dark green), C2 (red), D2 (mustard yellow), D3 (dark purple), E (black), F1 (grey), F2 (light green), G (orange). The red square represents the reference sequence.

DOI: 10.7287/peerj.preprints.3087v1/supp-2

Median network of horse haplotypes

Included are those of the 285 Hungarian draft individuals analysed in this study, plus sequences of European breeds available in the Genbank nucleotide database. Sectors are proportional to the frequency of each haplotype. Horizontal bars represent the mutational steps. MH, Hungarian draft, our samples (number of samples: 285); Rin, Rhineland Heavy draft (25); Nori, Noriker (10); Tat, Turkoman Akhal Teke (19); Ital, Italian heavy draught (27); Bre, Breton (58); Arb, Arabian (10); Fin, Finn horse (2); PP, Polish primitiv (3); HC, Hucul (10); Zem, Zemaitukai heavy type (7); Shi, Shire (10); Vlad, Vladimir draught horse (21); Cly, Clydesdale (17); Irn, Iranian (14); Tra, Trakehner (4); Csp, Caspian Pony (5); Ph, Polish heavy (3); Mrn, Maremmano (15); Pos, Posavina (20); Akt, Akhal teke (16); Shet, Shetland pony (12); Pur, Pura Raza Espanola (17); Hhv, Croatian heavy draft (11); Mur, Murinsulaner (8); Fell, Fell (2); Ic, Icelandic Horse (2); Nf, Norwegian Fjord (2); Rom, Romanian draft horse (1); Per, Percheron (3); Lhd, Lithuanian heavy drought (3); Old, Oldenburg (1); And, Andalusian (2); Sil, Silesian (1); Ital, Italian (3); Sc, Scottish Highland (2); Be, Belgian (13); Tho, Thoroughbred horse (1); Aph, American Paint horse (1); Han, Hanovarian (3); Wiel, Wielkopolski (3); Syr, Syrian (5); Gia, Giara horse (2); His, Holstein (2); Prze, Przewalskii (3); Got, Gotland (3); Sf, Suffolk Punch (1); West, Westfalian (1); Chin, Chincoteague pony (1); Cle, Cleveland bay horse (11); Sad, Saddlebred (1); Ex, Exmoor pony (1).

DOI: 10.7287/peerj.preprints.3087v1/supp-3

Matrix of pairwase FST values

Significance level=0.05.

DOI: 10.7287/peerj.preprints.3087v1/supp-4

Polymorphic sites in the control region of the Hungarian draft horse population sequenced

Nucleotide positions 15531–15752 as compared to GenBank reference sequence X79547 [11]. Sequence identity is indicated by ‘.’, gaps by ‘-‘.

DOI: 10.7287/peerj.preprints.3087v1/supp-5

Number of sequenced individuals (n), total number of haplotypes and polymorphic sites with their dispersion within 52 different horse populations

DOI: 10.7287/peerj.preprints.3087v1/supp-6

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Nikolett Csizmár conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables.

Sándor Mihók conceived and designed the experiments, reviewed drafts of the paper, reagents/materials/analysis tools.

András Jávor conceived and designed the experiments, reviewed drafts of the paper, reagents/materials/analysis tools.

Szilvia Kusza conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, reviewed drafts of the paper, reagents/materials/analysis tools.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

The following information was supplied relating to ethical approvals (i.e., approving body

and any reference numbers):

All horses in this study were client-owned on which no harmful invasive procedure was

performed; and there was no animal experimentation according to the legal definitions in

Europe.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

The following information was supplied regarding the deposition of DNA sequences: GenBank accession numbers of D-loop haplotypes: KY512807 to KY51309, it is not publicly available yet.

Funding

The study was supported by the Hungarian Draft Horse Breeders Association. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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