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The Ebola virus, a negative-sense single-stranded RNA virus, causes severe viral hemorrhagic fever and is highly lethal. Histopathology and immunopathologic study of Ebola virus have revealed that histopathologic changes in skin tissue were mainly various degrees of endothelial cell swelling and necrosis. The interactions of microbes within or on a host are a crucial aspect of the skin immune shield. The discovery of microRNAs in Ebola virus implies that immune escape, endothelial cell rupture and tissue dissolution during Ebola virus infection are all results of the action of Ebola virus miRNAs. Keratinocytes obtained from normal skin and subsequently attached and spread on the thrombospondin protein family may play a role in initiating cell-mediated immune responses in the skin. Several miRNAs have been observed to bind the 3' untranslated region of the thrombospondin mRNA, thereby controlling its stability and translational activity. In this study, we first discover short RNA sequences that might act as miRNAs from Propionibacterium acnes by design a practical workflow of bioinformatics methods. Subsequently, we deciphered the common target gene. These RNA sequences tend to binding to the same thrombospondin protein. These RNA sequences tend to bind to the same protein , THSD4, emphasizing the potential importance of the synergistic binding of miRNAs from Ebola virus, Propionibacterium acnes , and humans to the target. By RNA expression validation, we prove the potential synergistic binding of the miRNA from Ebola virus, Propionibacterium acnes and human to the target.
In this manuscript, we show the following results:
In this experiment, we revealed that PA-mir-2-3p-5 might be a very short RNA sequence but it could also behave as a miRNA.
Transfection of two different short RNAs together could also increase the expression of THSD4 mRNA better than transfection miRNA alone.
The mRNAs tend to contain several binding sites for the miRNA, emphasizing the potential importance of synergistic binding of the miRNA to the target. We believe that this manuscript is appropriate for publication by Peer J because it provides biologists possible way to access bioinformatics tools for future studies.