The isolation and identification of pathogenic fungus from the diseased  Tessaratomapapillosa  Drury (Hemiptera: Tessaratomidae) and their pathogenicity

Xiang Meng; Junjie Hu; Gecheng Ouyang

doi:10.7287/peerj.preprints.2851v1

The isolation and identification of pathogenic fungus from the diseased Tessaratomapapillosa Drury (Hemiptera: Tessaratomidae) and their pathogenicity

Xiang Meng ¹, Junjie Hu², Gecheng Ouyang¹

1 Guangdong Institute of Applied Biological Resources, Guangzhou, Guangdong, China

2 College of Life Science, Guangzhou University, Guangzhou, Guangdong, China

DOI: 10.7287/peerj.preprints.2851v1

Published: 2017-03-04
Accepted: 2017-03-04

Subject Areas: Agricultural Science, Entomology, Microbiology, Toxicology
Keywords: Tessaratoma papillosa Drury, entomopathogenic fungi, biological control, bioactivity

Copyright: © 2017 Meng et al.
Licence: This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.

Cite this article: Meng X, Hu J, Ouyang G. 2017. The isolation and identification of pathogenic fungus from the diseased Tessaratomapapillosa Drury (Hemiptera: Tessaratomidae) and their pathogenicity. PeerJ Preprints 5:e2851v1 https://doi.org/10.7287/peerj.preprints.2851v1

Abstract

Background. Litchi stink-bug, Tessaratoma papillosa Drury (Hemiptera: Tessaratomidae) is a major pest on litchi and longan in Southern China. It is urgent to develop valid biological agent for control the pest and improve IPM strategy on orchard farming. Entomopathogenic fungi was regarded as avital ecological factor pressing pest populations in field, however, there were fewer searches conducted on entomopathogenic fungi against litchi stink-bug. Methods & Results. In this study, two pathogenic fungus were isolated from the adult diseased T. papillosa by normal methods and rDNA-ITS homogeneous analysis, they are identified as Paecilomyces lilacinus and Beauveria bassiana. Laboratory tests showed that the two entomopathogenic fungi both had a good lethal effect to young nymph and old nymph of T. papillosa. The toxicity determination showed that the LC50 value for Beauveria bassiana was higher than P. lilacinus. Conclusion.These pathogenic fungus did not have the risk of pollution or residue, and they can be a alternative option for integrated pest management approache.