1 Citation   Views   Downloads

Bone morphogenetic protein 15 induces differentiation of mesenchymal stem cell derived from human follicular fluid to oocyte like cell

View preprint
37 days ago
Bone morphogenetic protein 15 induces differentiation of mesenchymal stem cell derived from human follicular fluid to oocyte like cell https://t.co/e2YIiUYrSY https://t.co/gMs7h5put4
NOT PEER-REVIEWED
"PeerJ Preprints" is a venue for early communication or feedback before peer review. Data may be preliminary.

Supplemental Information

DNA synthesis by BrdU incorporation

Graph pad prism 6 data analysis method

DOI: 10.7287/peerj.preprints.28006v1/supp-1

Cover letter

All authors have approved the manuscript and agree with its submission to PeerJ.

DOI: 10.7287/peerj.preprints.28006v1/supp-2

ELISA for estradiol assays

Determination of estradiol production in medium within process of differentiation, by graph pad prism 6 software.

DOI: 10.7287/peerj.preprints.28006v1/supp-3

Graph analysis

cytoplasmic immunostaining for vimentin analysis by graphpad prism6.

DOI: 10.7287/peerj.preprints.28006v1/supp-4

RT-PCR raw data

CT number of amplification plot for ZP3

DOI: 10.7287/peerj.preprints.28006v1/supp-5

RT-PCR

CT number of amplification plot for ZP2 and ZP3 weak 1.

DOI: 10.7287/peerj.preprints.28006v1/supp-6

RT-PCR- raw data

CT number of treated group.ZP3 and Nanog

DOI: 10.7287/peerj.preprints.28006v1/supp-7

RT-PCR raw data

CT number zp3 waek 3.

DOI: 10.7287/peerj.preprints.28006v1/supp-8

RT-PCR raw data

CT numbers of ZP2 control group.

DOI: 10.7287/peerj.preprints.28006v1/supp-9

RT-PCR raw data

CT numbers of OCT4 and Nanog after treatment with induction medium.

DOI: 10.7287/peerj.preprints.28006v1/supp-10

Sampling the ovarian follicular fluids

Hypo-osmotic lysis technique was used to enrich the follicular cells and elimination of red blood cells from FF.

DOI: 10.7287/peerj.preprints.28006v1/supp-11

Adipogenic differentiation of human FF cells. Raw dat

Oil Red O staining

DOI: 10.7287/peerj.preprints.28006v1/supp-12

Osteoinduction prestaining

osteogenic differentiation of human FF cells. Von Kossa staining of follicular fluid derived cells with differentiation medium during three weeks

DOI: 10.7287/peerj.preprints.28006v1/supp-13

Adipogenic media .Raw data

before staining procedure

DOI: 10.7287/peerj.preprints.28006v1/supp-14

Cell morphology raw data

Primary cell culture of FF planted on plate .

DOI: 10.7287/peerj.preprints.28006v1/supp-15

cell morphology.Raw data

fibroblast-like shape.

DOI: 10.7287/peerj.preprints.28006v1/supp-16

Cell morphology after treatment

morphology of FF derived cells culture in medium supplemented with BMP15

DOI: 10.7287/peerj.preprints.28006v1/supp-17

cell morphology

figurevshow different size of germ cell and OLCs developed spontaneously from human FF derived cells.

DOI: 10.7287/peerj.preprints.28006v1/supp-18

DAPI stainig.raw data

Pluripotent activities of human FF-derived cells

DOI: 10.7287/peerj.preprints.28006v1/supp-19

FITC raw data

Pluripotent activities of human FF-derived cells

DOI: 10.7287/peerj.preprints.28006v1/supp-20

pluripotency markers of OCT4

FITC

DOI: 10.7287/peerj.preprints.28006v1/supp-21

DAPI for OCT4.Raw data

pluripotency markers of OCT4

DOI: 10.7287/peerj.preprints.28006v1/supp-22

cytoplasmic immunostaining for vimentin

Raw data.

DOI: 10.7287/peerj.preprints.28006v1/supp-23

cytoplasmic immunostaining for vimentin.Raw data

vimentin immunostaining of FF derived adherent cells

DOI: 10.7287/peerj.preprints.28006v1/supp-24

vimentin ICC of FF derived adherent cells

DAPI and FITC staining.

DOI: 10.7287/peerj.preprints.28006v1/supp-25

OLC morphology raw data

BMP treated group.

DOI: 10.7287/peerj.preprints.28006v1/supp-26

DAPI OLC

after treatment with BMP15, using germ cell marker immunolocalization.

DOI: 10.7287/peerj.preprints.28006v1/supp-28

OLC for FITC

after treatment with BMP15 using germ cell marker immunolocalization

DOI: 10.7287/peerj.preprints.28006v1/supp-29

OLC for DAPI

after treatment with BMP15 using germ cell marker immunolocalization

DOI: 10.7287/peerj.preprints.28006v1/supp-30

Control negative

Control negative for using germ cell marker immunolocalization. data not shown in main document.

DOI: 10.7287/peerj.preprints.28006v1/supp-31

DAPI for negative control

the nuclei were counterstained and observed by 4′,6-Diamidino-2-phenylindole (DAPI)

DOI: 10.7287/peerj.preprints.28006v1/supp-32

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Mahin Taheri Moghadam conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Ali Reza Eftekhari Moghadam conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Ghasem Saki performed the experiments, contributed reagents/materials/analysis tools.

Roshan Nikbakht performed the experiments, contributed reagents/materials/analysis tools, sample collection and ovarian puncture.

Human Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

The Ethic committee of Ahvaz Jundishapur University of Medical Science has approved the study on August 17, 2017(IR.AJUMS.REC.1396.443). All patients provided a consent form based on the Helsinki Declaration.

Data Deposition

The following information was supplied regarding data availability:

Data submitted as supplementary file.

Funding

The current study was funded by the Research Deputy of Ahvaz Jundishapur University of Medical Science, Ahvaz, Iran (grant number of CMRC – 9616). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


Add your feedback

Before adding feedback, consider if it can be asked as a question instead, and if so then use the Question tab. Pointing out typos is fine, but authors are encouraged to accept only substantially helpful feedback.

Some Markdown syntax is allowed: _italic_ **bold** ^superscript^ ~subscript~ %%blockquote%% [link text](link URL)
 
By posting this you agree to PeerJ's commenting policies