Immunocytological analysis of chromosomes in meiotic prophase I of the paleotetraploid frog Xenopus laevis
- Published
- Accepted
- Subject Areas
- Evolutionary Studies, Genetics, Zoology
- Keywords
- Xenopus laevis, frog, synaptonemal complex, prophase I, meiotic chromosomes, SYCP3, Rad51, DSB, synapsis, homeolog
- Copyright
- © 2019 Matveevsky et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
- Cite this article
- 2019. Immunocytological analysis of chromosomes in meiotic prophase I of the paleotetraploid frog Xenopus laevis. PeerJ Preprints 7:e27937v1 https://doi.org/10.7287/peerj.preprints.27937v1
Abstract
The African clawed frog Xenopus laevis (Pipidae, Anura, Amphibia) is a model object of cell and evolutionary biology. X. laevis is a paleotetraploid frog. For the first time, the results of an immunocytological analysis of the Xenopus chromosome behaviour in the meiotic prophase I based on synaptonemal complexes (SC) are presented. We identified all stages of meiosis prophase I, and determined the dynamics of the main proteins involved in the synapsis and repair of DNA DSBs. We did not find a homeologous synapsis.
Author Comment
This is a preprint submission to PeerJ
Supplemental Information
Fig.1
Xenopus laevis spermatocytes at different stages of prophase I. a. Zygotene stage. Chromosome bouquet; b. Pachytene stage; c. Diplotene. White signal corresponds to the distribution of the major protein of SC - SYCP3. Magnification × 1000.
Fig.2
Representation of chromosome synapsis in Xenopus. Chromosome telomeres are grouped near one of the nucleus poles at the zygotene (“bouquet” stage). In pachytene, SCs are formes by homologs. Homeologous synapsis is absent.
