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A new primer for metabarcoding of spider gut contents

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RT @VascoElbrecht: Looking for spider gut content #metabarcoding primers? @ResearchDenis @JordanCuff @dirch3 @PeterHamback Ann & I develope…
59 days ago
RT @VascoElbrecht: Looking for spider gut content #metabarcoding primers? @ResearchDenis @JordanCuff @dirch3 @PeterHamback Ann & I develope…
RT @JordanCuff: The preprint for NoSpi2, the new spider gut content metabarcoding primer with @ResearchDenis @VascoElbrecht @dirch3 @PeterH…
61 days ago
RT @JordanCuff: The preprint for NoSpi2, the new spider gut content metabarcoding primer with @ResearchDenis @VascoElbrecht @dirch3 @PeterH…
RT @JordanCuff: The preprint for NoSpi2, the new spider gut content metabarcoding primer with @ResearchDenis @VascoElbrecht @dirch3 @PeterH…
The preprint for NoSpi2, the new spider gut content metabarcoding primer with @ResearchDenis @VascoElbrecht @dirch3 @PeterHamback & Ann Erlandsson is now out! Really exciting being part of such an international project with such talented scientists!
RT @VascoElbrecht: Looking for spider gut content #metabarcoding primers? @ResearchDenis @JordanCuff @dirch3 @PeterHamback Ann & I develope…
RT @VascoElbrecht: Looking for spider gut content #metabarcoding primers? @ResearchDenis @JordanCuff @dirch3 @PeterHamback Ann & I develope…
RT @ResearchDenis: You are interested in #spider #diet, #biocontrol, #foodwebs ? We have developed a primer for spider gut content #metabar…
68 days ago
RT @VascoElbrecht: Looking for spider gut content #metabarcoding primers? @ResearchDenis @JordanCuff @dirch3 @PeterHamback Ann & I develope…
RT @ResearchDenis: You are interested in #spider #diet, #biocontrol, #foodwebs ? We have developed a primer for spider gut content #metabar…
RT @VascoElbrecht: Looking for spider gut content #metabarcoding primers? @ResearchDenis @JordanCuff @dirch3 @PeterHamback Ann & I develope…
RT @ResearchDenis: You are interested in #spider #diet, #biocontrol, #foodwebs ? We have developed a primer for spider gut content #metabar…
RT @VascoElbrecht: Looking for spider gut content #metabarcoding primers? @ResearchDenis @JordanCuff @dirch3 @PeterHamback Ann & I develope…
RT @VascoElbrecht: Looking for spider gut content #metabarcoding primers? @ResearchDenis @JordanCuff @dirch3 @PeterHamback Ann & I develope…
69 days ago
A new primer for metabarcoding of spider gut contents https://t.co/EfGaUy1tkm https://t.co/96CQZJTvEr
69 days ago
RT @VascoElbrecht: Looking for spider gut content #metabarcoding primers? @ResearchDenis @JordanCuff @dirch3 @PeterHamback Ann & I develope…
69 days ago
RT @ResearchDenis: You are interested in #spider #diet, #biocontrol, #foodwebs ? We have developed a primer for spider gut content #metabar…
A new primer for metabarcoding of spider gut contents https://t.co/anDIw36wxn https://t.co/23u1xqbO1o
A new primer for metabarcoding of spider gut contents https://t.co/GQV7jiX4nU
RT @ResearchDenis: You are interested in #spider #diet, #biocontrol, #foodwebs ? We have developed a primer for spider gut content #metabar…
Looking for spider gut content #metabarcoding primers? @ResearchDenis @JordanCuff @dirch3 @PeterHamback Ann & I developed #NoSpi2: It fully excludes selected predators (0 reads) while showing a good recovery of pray insects. Read more here @PeerJPreprints: https://t.co/bkVfnlJnU2 https://t.co/ei0GqEGm1f
69 days ago
You are interested in #spider #diet, #biocontrol, #foodwebs ? We have developed a primer for spider gut content #metabarcoding amplifying all potential prey !! Check our preprint @PeerJPreprints https://t.co/MBUJffGqVG @VascoElbrecht @JordanCuff @dirch3 @PeterHamback
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Supplemental Information

Summary of the spiders tested

DOI: 10.7287/peerj.preprints.27854v1/supp-2

Picture of the gel electrophoresis obtained after PCR with NoSpi2/BR2

Coll: Collembola (Poduridae), Trico1: Trichoptera (Limnephiliidae), Trico2: Trichoptera (Phryganeidae), Trico3: Trichoptera (Polycentropodidae), Pleco: Plecoptera (Nemouridae), Carab1: Carabidae (Bembidion bruxellens), Neg: negative control

DOI: 10.7287/peerj.preprints.27854v1/supp-3

Picture of the gel electrophoresis obtained after PCR with NoSpi2/BR2

Eph1: Ephemeroptera (Baetidae), Eph2: Ephemeroptera (Leptoplebiidae), Eph3: Ephemeroptera (Leptoplebiidae), Megalo: Megaloptera, Carab2: Carabidae (Bembidion sp.), Carab3: Carabidae (Elaphrus riparius), Pardo abdo: starved Pardosa abdomen

DOI: 10.7287/peerj.preprints.27854v1/supp-4

Picture of the gel electrophoresis obtained after PCR with NoSpi2/BR2

Dipt1: Dipatera (Chironomidae), Dipt2: Diptera (Chaboridae), Dipt3: Diptera (Simulidae), Dipt4: Diptera (Simulidae), Pardo1: Lycosidae (Pardosa amentata), Pardo2: Lycosidae (Pardosa lugubris), Pardo+Coll: starved Pardosa + collembola, Neg: Negative control

DOI: 10.7287/peerj.preprints.27854v1/supp-5

Picture of the gel electrophoresis obtained after PCR with NoSpi2/BR2

DOI: 10.7287/peerj.preprints.27854v1/supp-6

Picture of the gel electrophoresis obtained after PCR with NoSpi2/BR2

Pardo3: Lycosidae (Pardosa prativaga), Pardo4: Lycosidae (Pardosa pullata), Pirat: Lycosidae (Piratula hygophila), Tetra: Tetragnathidae (Tetragnatha sp.), Liny1: Linyphiidae (Tenuiphantes cristatus), Liny2: Linyphiidae (Bathyphantes nigrinus), Pardo+Tricho: starved Pardosa + Trichoptera, Pardo + Pleco: starved Pardosa + Plecoptera

DOI: 10.7287/peerj.preprints.27854v1/supp-7

Picture of the gel electrophoresis obtained after PCR with NoSpi2/BR2

1: Thomisidae (Diaea dorsata), 2: Theridiidae (Enoplognatha ovata), 3: Araneidae (Araneus alsine), 4: Pisauridae (Pisaura mirabilis), 5: Hahniidae (Hahnia nava), 6: Cybaeidae (Cryphoeca sylvicola), 7: Tetragnathidae (Metellina merianae) , 8: Salticidae (Neon reticulatus) , 9: Oxyopidae (Oxyopes salticus), 10: Ctenidae, Neg: negative control

DOI: 10.7287/peerj.preprints.27854v1/supp-8

Summary table of all the species detected in spider gut contents during the field test

# detect: number of spiders in which a prey was detected. # reads: total number of reads in the library. Sequences were attributed at species level with a similarity match ≥ 98%, to the genus level with a similarity match ≥ 95%, to the family level with a similarity match ≥ 90% and to the order level with a similarity match ≥ 85%.

DOI: 10.7287/peerj.preprints.27854v1/supp-9

Raw amplicon sequence variant table from field test

DOI: 10.7287/peerj.preprints.27854v1/supp-10

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Denis Lafage conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Vasco Elbrecht conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Jordan Cuff performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

Dirk Steinke contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

Peter Hambäck conceived and designed the experiments, authored or reviewed drafts of the paper, approved the final draft.

Ann Erlandsson conceived and designed the experiments, performed the experiments, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

Trimmed sequences from the Sanger sequencing (in vitro evaluation) are available under the NOSPI project on BOLD.

Raw sequence data from the mock community metabarcoding and malaise sample are available under the SRA accession numbers SRX5975749 and SRX5975748. Data from the complete run with the 96 additional samples not analysed in this study is available under accession PRJNA546583.

Raw sequence data from the field test metabarcoding is available under SRA accession number PRJNA550981.

Data Deposition

The following information was supplied regarding data availability:

The code used for data analysis is available on GitLab under the project ID: 9419382 (https://gitlab.com/DenisLafage/nospi)

Funding

The RIVEM group (Karlstad University) funded DL and the in vitro and field tests. V.E. and D.S. as well as the metabarcoding experiments were supported by funding through the University of Guelph’s Canada First Research Excellence Fund project Food from Thought. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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