Study of the optimizing karyotype by applying AC voltage

Amir Shams; Hoda Ayat; Ali Mohammad Ahadi

doi:10.7287/peerj.preprints.27776v1

Study of the optimizing karyotype by applying AC voltage

Amir Shams ^1,2, Hoda Ayat², Ali Mohammad Ahadi²

1 Stem cell and Genetics Department, Royan Biotechnology Institute, Isfahan, Esfahan, IRAN

2 Basic Science Facility, Genetic Department, Shahrekord University, Shahrekord, Chaharmahal and Bhatiyari, IRAN

DOI: 10.7287/peerj.preprints.27776v1

Published: 2019-06-02
Accepted: 2019-06-02

Subject Areas: Genetics, Science and Medical Education, Medical Genetics
Keywords: Cytogenetics, Karyotype, Chromosome Slide, Ramp Voltage, AC Voltage

Copyright: © 2019 Shams et al.
Licence: This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.

Cite this article: Shams A, Ayat H, Ahadi AM. 2019. Study of the optimizing karyotype by applying AC voltage. PeerJ Preprints 7:e27776v1 https://doi.org/10.7287/peerj.preprints.27776v1

Abstract

Background: The main problem in the conventional karyotype slide is twisted and folded chromosomes that make it challenging to analyze the karyotype slide. Most of the time karyotype sorting software mistake chromosome with each order. In this study, the alternating current (AC) voltage conducted in order to drag the chromosome on karyotype slides. This method can facilitate the analysis of chromosomes structure by researchers, and karyotype sorting software can perform better and show a more accurate result.

Methods: In this study, the AC ramp voltage has been applied to drag the chromosome on karyotype slides. AC voltage interacts with chromosomes in the slide so the force of electric charge can untwist and unfold chromosomes partially.

Results: Karyotype chromosomes that were under AC voltage showed somewhat lined, and arranged chromosomes compare to conventional karyotype chromosomes.

Conclusions: This method makes the study of the chromosomes structure very easy and more reliable. Hopefully, with the design and development of custom signal generators, the results of the study will be better and more accurate.

Author Comment

The same procedure to isolate and analyze chromosomes for karyotype used for decades. In this study, we try to improve the karyotype basic protocol to make better and more accurate karyotype slides . we use low-frequency voltage to interact with chromosomes and move them back and forward.