Off-line and on-line optical monitoring of microalgal growth
A peer-reviewed article of this Preprint also exists.
Author and article information
Abstract
The growth of Chlamydomonas reinhardtii microalgae cultures was successfully monitored, from classic off-line optical techniques (optical density and fluorescence) to on-line analysis of digital images. In this study, it is shown that the chlorophyll fluorescence ratio F685/F740 has a linear correlation with the logarithmic concentration of microalgae. Moreover, with digital images, the biomass concentration was correlated with: the luminosity of the images through an exponential equation, and the length of penetration of a superluminescent blue beam (λ=440 nm), through an inversely proportional function. Outcomes of this study are useful to monitor both research and industrial microalgae cultures.
Cite this as
2019. Off-line and on-line optical monitoring of microalgal growth. PeerJ Preprints 7:e27744v1 https://doi.org/10.7287/peerj.preprints.27744v1note This preprint is not peer-reviewed. You may wish to reference the subsequent peer-reviewed version of this article.
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This is a submission to PeerJ for review.
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Supplemental Information
Of-line fluorescence measurements of C. reinhardtii cultures
raw data of: Of-line fluorescence measurements of C. reinhardtii cultures, corresponding to experiments A and B
Of-line optical density measurements of C. reinhardtii cultures
Raw data of: Of-line optical density measurements of C. reinhardtii cultures, corresponding to experiments A and B
Luminosity and bean penetration measurements in PBR
Raw data of Luminosity and Optical density vs bean penetration measurements in PBR.
Additional Information
Competing Interests
The authors declare that they have no competing interests.
Author Contributions
Hugo-Enrique Lazcano-Hernandez conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft, redesign of experiments and analysis.
Gabriela Aguilar performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, approved the final draft.
Gabriela Dzul performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, approved the final draft.
Rodrigo Patiño conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.
Javier Arellano-Verdejo analyzed the data, prepared figures and/or tables, approved the final draft.
Data Deposition
The following information was supplied regarding data availability:
The raw measurements are provided in the supplementary files 1
Funding
The authors received no funding for this work.
