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Supplemental Information

Predicted amino acid sequences for FvJAZ1/8

DOI: 10.7287/peerj.preprints.2772v1/supp-1

Identity among COI1, JAZ1 and JAZ8 ortholog proteins from A. thaliana V. vinifera, S. lycopersicum and M. ×domestica with respect to F. vesca proteins

DOI: 10.7287/peerj.preprints.2772v1/supp-2

Validation of FvCOI1, FvJAZ1 and FvJAZ8 structures using the PROCHECK program (Ramachandran plot)

DOI: 10.7287/peerj.preprints.2772v1/supp-3

Alignment of the degron amino acid sequences from FvJAZ1 and JAZ1 orthologs

Gaps are indicated by dashes, letters with black background represent identical amino acid residues. GenBank accession numbers are showed in parentheses: FvJAZ1 (Fragaria vesca JAZ1, XP_004287655), AtJAZ1, AtJAZ2.1, AtJAZ3.1, AtJAZ4, AtJAZ5, AtJAZ6, AtJAZ7, AtJAZ8, AtJAZ9, AtJAZ10.1, AtJAZ11, and AtJAZ12 (Arabidopsis thaliana JAZ1-12, NP_564075, NP_565096, NP_566590, NP_175283, NP_564019, NP_565043, NP_181007, NP_564349, NP_177227, NP_568287, NP_189930, and NP_197590, respectively), PpJAZ1 (Prunus persica JAZ1, XP_007202425.1), PmTIFY10A (Prunus mume TIFY10A, XP_008242268.1), PbTIFY10A (Pyrus ×bretschneideri TIFY10A, XP_009337185.1), PbTIFY10A-like (Pyrus ×bretschneideri TIFY10-like, XP_009359716.1), MdTIFY10A (Malus ×domestica TIFY10A, (XP_008388962.1), MdTIFY10A-like (Malus ×domestica TIFY10A-like, XP_008337828.1), MnTIFY10A (Morus notabilis TIFY10A, XP_010099096.1), ZjTIFY10B (Ziziphus jujuba TIFY10B, XP_015889049.1), ZjTIFY10A (Ziziphus jujuba TIFY10A, XP_015889048.1), CfTIFY10A (Cephalotus follicularis TIFY10A, GAV76247.1), PeTIFY10A (Populus euphratica TIFY10A, XP_011039955.1), CsTIFY10A (Citrus sinensis TIFY10A, XP_006474700.1), CrJAZ1 (Catharanthus roseus JAZ1, ACM89457.1), HbJAZ1 (Hevea brasiliensis JAZ1, ADI39634.1), CsJAZ1 (Camellia sinensis JAZ1, ANB66340.1), LaTIFY10B (Lupinus angustifolius TIFY10B, XP_019443429.1), VvJAZ1 (Vitis vinifera JAZ1, CBI27776.3), RcTIFY10A (Ricinus communis TIFY10A, XP_002516243.1), GhTIFY10A-like (Gossypium hirsutum TIFY10A, XP_016682369.1), GrTIFY10A-like (Gossypium raimondii TIFY10A, XP_012451673.1), VrJAZ1 (Vitis rupestris JAZ1, AEP60132.1), VvTIFY10A (Vitis vinifera TIFY10A, XP_002277157.1), TcTIFY10A (Theobroma cacao TIFY10A, EOY30213.1), EcJAZ1 (Eleusine coracana JAZ1, KJ689791).

DOI: 10.7287/peerj.preprints.2772v1/supp-4

Structural superposition of FvCOI11 structural model with AtCOI1 used as template

(A) Front view of the structure. (B) Top view of the structure. In red and grey are represented the template and the FvCOI1 structural models, respectively.

DOI: 10.7287/peerj.preprints.2772v1/supp-5

FvJAZ1 and FvJAZ8 structural models

(A) Schematic representation and alignment close up of Jas domains from AtJAZ1, FvJAZ1, and FvJAZ8. (B) FvJAZ1 structural model. (C) FvJAZ8 structural model. (D) Superposition of FvJAZ1 and FvJAZ8 structural models with AtJAZ1. In red, blue and orange is shown the template, FvJAZ1 and FvJAZ8, respectively. (E)Structural superposition of FvJAZ1 and FvJAZ8 structural models.

DOI: 10.7287/peerj.preprints.2772v1/supp-6

RMSD of each protein-ligand-protein complex obtained during 100 ns of molecular dynamics simulations (MDS)

In red is represented the complex formed between FvCOI1-JA-Ile and FvJAZ8, in purple the complex formed between FvCOI1-JA and FvJAZ1, in green the complex formed between FvCOI1-JA-Ile and FvJAZ1, and in blue the complex formed between FvCOI1-JA-Ile and FvJAZ8.

DOI: 10.7287/peerj.preprints.2772v1/supp-7

Interaction mode of the FvCOI1-JA-Ile-FvJAZ1 complex obtained during a 100 ns of molecular dynamics simulations (MDS)

DOI: 10.7287/peerj.preprints.2772v1/supp-8

Interaction mode of the FvCOI1-JA-FvJAZ1 complex obtained during a 100 ns of molecular dynamics simulations (MDS)

DOI: 10.7287/peerj.preprints.2772v1/supp-9

Interaction mode of the FvCOI1-JA-Ile-FvJAZ8 complex obtained during a 100 ns of molecular dynamics simulations (MDS)

DOI: 10.7287/peerj.preprints.2772v1/supp-10

Interaction mode of the FvCOI1-JA-FvJAZ8 complex obtained during a 100 ns of molecular dynamics simulations (MDS)

DOI: 10.7287/peerj.preprints.2772v1/supp-11

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Felipe Valenzuela-Riffo performed the experiments, analyzed the data, prepared figures and/or tables.

Adrián Garrido-Bigotes analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Pablo M Figueroa conceived and designed the experiments, analyzed the data, wrote the paper, reviewed drafts of the paper.

Luis Morales-Quintana conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper, got research fund.

Carlos R Figueroa conceived and designed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper, got research fund.

Data Deposition

The following information was supplied regarding data availability:

www.rosaceae.org

Funding

This work was supported by the National Commission for Technological and Scientific Research (CONICYT) of Chile [grants CONICYT, FONDECYT/Regular 1140663 to C.R.F. and CONICYT PAI/ACADEMIA 79140027 to L.M-Q.]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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