Development of genomic simple sequence repeat markers for Glycyrrhiza  lepidota  and cross-amplification of other  Glycyrrhiza  species

Jun Hyoung Bang; Chi Eun Hong; Sebastin Raveendar; Kyong Hwan Bang; Kyung Ho Ma; Soon Wook Kwon; Hojin Ryu; Ick Hyun Jo; Jong Wook Chung

doi:10.7287/peerj.preprints.27690v1

Development of genomic simple sequence repeat markers for Glycyrrhiza lepidota and cross-amplification of other Glycyrrhiza species

Jun Hyoung Bang¹, Chi Eun Hong², Sebastin Raveendar³, Kyong Hwan Bang², Kyung Ho Ma², Soon Wook Kwon⁴, Hojin Ryu⁵, Ick Hyun Jo ², Jong Wook Chung ¹

1 Department of Industrial Plant Science and Technology, Chungbuk National University, Cheongju, South Korea

2 Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Eumseong, South Korea

3 National Agrobiodiversity Center, National Institute of Agricultural Science, Jeonju, South Korea

4 Department of Plant Bioscience, Pusan National University, Miryang, South Korea

5 Department of Biology, Chungbuk National University, Cheongju, South Korea

DOI: 10.7287/peerj.preprints.27690v1

Published: 2019-04-29
Accepted: 2019-04-29

Subject Areas: Agricultural Science, Biodiversity, Conservation Biology, Plant Science, Taxonomy
Keywords: Cross-amplification, Genetic diversity, Licorice, Simple sequence repeat marker, Transferability

Copyright: © 2019 Bang et al.
Licence: This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.

Cite this article: Bang JH, Hong CE, Raveendar S, Bang KH, Ma KH, Kwon SW, Ryu H, Jo IH, Chung JW. 2019. Development of genomic simple sequence repeat markers for Glycyrrhiza lepidota and cross-amplification of other Glycyrrhiza species. PeerJ Preprints 7:e27690v1 https://doi.org/10.7287/peerj.preprints.27690v1

Abstract

Background. Licorice (Glycyrrhiza spp. L.) is used as a natural sweetener and medicinal herb. Molecular studies have been conducted to find differences between wild and cultivated species because most wild species are highly resistant to abiotic and biotic stresses compared with their cultivated counterparts. However, few molecular markers have been developed for studying the genetic diversity and population structure of licorice species and to identify differences between cultivars. Thus, the present study aimed to develop a set of genomic simple sequence repeat (SSR) markers for molecular studies of these species.

Methods. We designed 100 SSR markers based on the whole-genome sequence data of wild Glycyrrhiza lepidota and selected 62 SSR markers.

Results. The genetic diversity analysis using these markers identified 2–23 alleles, and the major allele frequency, observed heterozygosity, genetic diversity, and polymorphism information content were 0.11–0.91, 0–0.90, 0.17–0.94, and 0.15–0.93, respectively. Interspecies transferability values were 93.5%, 91.6%, and 91.1% for G. echinata, G. glabra, and G. uralensis, respectively. Phylogenetic analysis clustered cultivated (group 1) and wild (group 2) species into three and two subgroups, respectively. The SSR markers developed here can be applied to genetic diversity, population structure, and cultivar differentiation studies, as well as to breeding of licorice varieties.