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Supplemental Information

Taxonomic distribution of OTUs at the family level

Relative abundance refers to percentage of the OTUs attributed to each family with respect to all OTUs from each sample, including those that were unclassified. Only families represented by a total of >0.1% of OTUs across all samples are shown. The five sample types are separated vertically by treatment (top two are effluent only and bottom three are river water or river with added effluent) and horizontally by time point.

DOI: 10.7287/peerj.preprints.27605v1/supp-1

Taxonomic distribution of OTUs at the genus level

Relative abundance refers to percentage of the OTUs attributed to each genus with respect to all OTUs from each sample, including those that were unclassified. Only genera represented by a total of >0.1% of OTUs across all samples are shown. The five sample types are separated vertically by treatment (top two are effluent only and bottom three are river water or river with added effluent) and horizontally by time point.

DOI: 10.7287/peerj.preprints.27605v1/supp-2

Predicted functional metagenomic pathways in effluent samples, as identified by PICRUSt and STAMP analyses

Functions are shown at hierarchical level 3 based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) Orthology (KO) database. Welch'st-test was used to calculate variance between untreated vs. UV-treated effluent samples, all time points combined. TheP-values and 95% confidence intervals for the total variance of the two groups areindicated for each function.

DOI: 10.7287/peerj.preprints.27605v1/supp-3

Differential abundances of specific taxa based on sample type and time (DeSeq)

Phylogenetic assignment is given to the deepest possible level up to family. BaseMean is the mean of normalized counts for all samples; padj is the Benjamini–Hochberg adjusted P value.

DOI: 10.7287/peerj.preprints.27605v1/supp-4

Differential abundance of sewage indicator bacteria in untreated and UV-treated effluent (DESeq)

Mean of normalized counts at each time point are shown; padj is the Benjamini–Hochberg adjusted P value.

DOI: 10.7287/peerj.preprints.27605v1/supp-5

Quantification of Cq results for bacterial amoA

DOI: 10.7287/peerj.preprints.27605v1/supp-6

Quantification of Cq results for rpoB

DOI: 10.7287/peerj.preprints.27605v1/supp-7

Quantification of Cq results for tetW

DOI: 10.7287/peerj.preprints.27605v1/supp-8

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Imrose Kauser conceived and designed the experiments, performed the experiments, analyzed the data.

Mark Ciesielski performed the experiments.

Rachel S Poretsky conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

All sequences have been deposited in the Sequence Read Archive under accession number SRP153092

Data Deposition

The following information was supplied regarding data availability:

The raw qPCR data Cq data for rpoB, amoA, and tetW are provided in supplementary files.

Funding

Funding was provided by the University of Illinois Chicago start-up funds and a grant from the UIC Honors College. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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